Protein Production

蛋白质生产

• 批准号: 8738012
• 负责人: Charles O Rock
• 金额: $ 0.08万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8738012
• 关键词: Baculoviruses; Cancer Center; Cancer Center Support Grant; Chemicals; Equipment; Funding; Human Resources; Molecular and Cellular Biology; Production; Proteins; Research Personnel; Resource Sharing; Services; Testing; Training; Translations; method development; protein expression; protein purification; screening; structural biology

The PPF equipment was acquired with institutional funds and Dr. Heath was hired to manage the facility in September, 2000. Two technologists were hired in January of 2001 and a third in July 2001. After an initial period of personnel training, equipment testing and method development, the shared resource was opened to Cancer Center investigators in July of 2001. Initially, services offered were bacterial expression, protein purification and protein crystallographic screening. In 2003, we began offering a baculovirus expression service, which has increased the usefulness to the Cancer Center investigators.

PPF 设备是通过机构资金购买的，并聘请 Heath 博士来管理该设施。 2000 年 9 月。2001 年 1 月聘用了两名技术人员，2001 年 7 月聘用了第三名技术人员。 人员培训、设备测试、方法开发期间，共享资源开放给 2001 年 7 月癌症中心调查人员。最初提供的服务是细菌表达、蛋白质 纯化和蛋白质晶体学筛选。 2003年，我们开始提供杆状病毒表达 服务，这增加了癌症中心研究人员的有用性。