Viral Vector

病毒载体

• 批准号: 10600158
• 负责人: PATRICK L SINN
• 金额: --
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-14 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10600158
• 关键词: Archives; Baculoviruses; Biological Assay; Cancer Center Support Grant; Catalogs; Cell Line; Consultations; Core Assembly; Country; Custom; Databases; Dependovirus; Development; Discipline; Education; Fertilization; Gene Expression; Gene Transfer; Generations; Genes; Guide RNA; Institution; Joints; Journals; Lentivirus; Malignant Neoplasms; Methods; NCI-Designated Cancer Center; Participant; Plasmids; Preparation; Process; Production; Program Development; Protocols documentation; Quality Control; Recombinants; Reporter; Research; Research Design; Research Personnel; Retroviridae; Services; Small Interfering RNA; Technology; Technology Transfer; Testing; Training; Transgenes; Update; Vaccinia; Viral; Viral Vector; anticancer research; base editing; delivery vehicle; design; expression vector; improved; innovation; meetings; member; new technology; novel; recombinant adenovirus; recombinant viral vector; research and development; research facility; vector; webinar

The Viral Vector Core (VVC) serves as a research facility that supports gene transfer studies. Its overall objective is to support investigators in the use of gene transfer technologies, including consultation with investigators, development of novel vectors, collaborative testing of vectors generated for function and purity, and preparation including quality control. The facility serves both HCCC members and a significant number of investigators from other NCI-designated cancer centers. VVC staff are active participants in the development of gene transfer technologies in the cancer field. The interaction with multiple investigators from various disciplines and institutions allows for cross-fertilization of ideas, technical advancements, and innovations in vector designs. The VVC provides purified and concentrated preparations of recombinant adenovirus, adeno-associated virus, vaccinia, baculovirus and retrovirus (including lentivirus). It also provides access to standard cell lines, expression plasmids and a stock of recombinant reporter viral vectors. The main responsibilities of the VVC are to: prepare recombinant vectors; perform relevant quality control; disseminate vectors; maintain a database of vector stocks available for use; maintain a database of expression vectors; develop new expression vectors as needed; develop novel methods for vector production; and assist in the design and development of novel vectors.

病毒载体核心 (VVC) 作为支持基因转移研究的研究设施。其总体 目标是支持研究人员使用基因转移技术，包括与 研究人员、新型载体的开发、针对功能和生成的载体的协作测试 纯度和制备，包括质量控制。该设施为 HCCC 会员和重要的 来自其他 NCI 指定癌症中心的研究人员数量。 VVC 员工积极参与 癌症领域基因转移技术的发展。与多个研究者的互动 来自不同学科和机构的人员可以相互交流思想、技术进步和 矢量设计的创新。 VVC 提供重组蛋白的纯化和浓缩制剂 腺病毒、腺相关病毒、牛痘、杆状病毒和逆转录病毒（包括慢病毒）。它还 提供标准细胞系、表达质粒和重组报告病毒库存 向量。 VVC的主要职责是： 制备重组载体；执行相关质量 控制;传播媒介；维护可供使用的病媒库存数据库；维护数据库 表达载体；根据需要开发新的表达载体；开发载体的新方法 生产;并协助设计和开发新型载体。