Molecular Analysis of Disease Pathways

疾病途径的分子分析

基本信息

批准号：
8507240
负责人：
Susan M Lunte
金额：
$ 211.61万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-15 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8507240
关键词：
Advertisements Animal Model Area Biochemical Biochemical Pathway Biological Biological Markers Biological Process Biomedical Engineering Biomedical Research Caenorhabditis elegans Cardiovascular Diseases Centers of Research Excellence Chemicals Chemistry Collaborations Core Facility Custom Development Devices Disease Disease Pathway Doctor of Philosophy Drug Delivery Systems Educational workshop Elements Facility Designs Faculty Fluorescent Probes Genes Genetic Genomics Goals Housing Image Immunoassay Institutes Institution Interdisciplinary Study Investigation Kansas Lung diseases Malignant Neoplasms Maps Mass Spectrum Analysis Mentors Methodology Methods Microfluidics Molecular Molecular Analysis Molecular Probes Monitor Neurosciences Organism Pathologic Processes Pathway interactions Pharmaceutical Chemistry Pharmacologic Substance Phenotype Physiologic Monitoring Process Publications Reagent Research Research Infrastructure Research Personnel Research Project Grants Science Scientist Structure System Technology Universities Zebrafish base chemical genetics chemical synthesis commercialization design empowered genetic manipulation implementation research improved in vivo innovation interest meetings mutant nervous system disorder new technology next generation novel professor programs protein function public health relevance screening symposium therapeutic target tool

项目摘要

DESCRIPTION (provided by applicant): The development of new enabling technologies is critical to improving our understanding and treatment of disease. Immunoassays, gene sequencing, and mass spectrometry represent examples of technologies that have revolutionized biomedical research by allowing the identification of biomarkers and disease pathways that could not have been discovered otherwise. We propose here a new COBRE center focused on Molecular Analysis of Disease Pathways. This Center will bring together junior and senior faculty from the physical, biological, and pharmaceutical sciences at the University of Kansas and other academic institutions in Kansas to conduct multidisciplinary research to develop and implement cutting-edge technologies for elucidating the genetic, chemical, and physical mechanisms of biological processes involved in disease. The scientific emphasis ofthe Center will be to create and implement new enabling technologies for identification of therapeutic targets. These enabling methodologies include methods for integrating next generation gene sequencing with genetic manipulation of model organisms, custom-synthesized fluorescent molecular probes for monitoring physiological/pathological processes in model organisms in vivo, and microfluidic systems for manipulation of model organisms and monitoring of biochemical pathways in vivo. A key innovative element of this proposal is the creation of three core facilities that are designed to operate synergistically: imaging of model organisms treated with fluorescent probes developed by one core facility will be facilitated by the use of microfabricated devices developed in a second core facility; screening of mutant organisms such as C. elegans and zebrafish against these probes will be used to discover novel disease-related phenotypes that can be precisely mapped to identify specific targets through the next generation genomic sequencing and related genomic technologies offered by a third core facility. The proposed Center will exploit the strengths of KU and associated universities in the areas of genetics, bioanalytical chemistry, bioengineering, and chemical synthesis and will create new methodologies and approaches that can be used to investigate any pathway involved in disease. These new technologies will be disseminated through research collaborations, publications, and potential commercialization.

描述（由申请人提供）：新启用技术的发展对于改善我们对疾病的理解和治疗至关重要。免疫测定，基因测序和质谱法代表了通过允许鉴定生物标志物和疾病途径彻底改变生物医学研究的技术的例子，这些途径否则无法发现。我们在这里提出了一个新的毛绒中心，重点是疾病途径的分子分析。该中心将汇集堪萨斯大学和堪萨斯州其他学术机构的物理，生物学和药学的初级和高级教职员工，以开发和实施尖端技术，以阐明涉及疾病的生物学过程的遗传，化学和物理机制，以开发和实施尖端的技术。该中心的科学重点是创建和实施新的启用技术来识别治疗靶标。这些能力方法包括将下一代基因测序与模型生物的遗传操纵，定制合成的荧光分子探针进行体内模型生物体中监测生理/病理过程的定制荧光分子探针以及微流体系统中的这些方法，用于对模型有机体的操纵和监测生物化学路径的操作。该提案的一个关键创新因素是创建旨在协同操作的三个核心设施：通过在第二个核心设施中开发的微型制造设备来促进用一个核心设施开发的荧光概率处理的模型生物的成像；对这些探针的突变生物（例如秀丽隐杆线虫和斑马鱼）的筛查将用于发现与疾病相关的新型表型，这些表型可以精确映射，以通过下一代基因组测序和相关的基因组技术来鉴定特定靶标。拟议的中心将利用KU的优势以及遗传学，生物分析化学，生物工程和化学合成领域的相关大学，并将创建新的方法和方法，可用于研究疾病涉及的任何途径。这些新技术将通过研究合作，出版物和潜在的商业化来传播。