Molecular Analysis of Disease Pathways

疾病途径的分子分析

基本信息

批准号：
8507240
负责人：
Susan M Lunte
金额：
$ 211.61万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-15 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8507240
关键词：
Advertisements Animal Model Area Biochemical Biochemical Pathway Biological Biological Markers Biological Process Biomedical Engineering Biomedical Research Caenorhabditis elegans Cardiovascular Diseases Centers of Research Excellence Chemicals Chemistry Collaborations Core Facility Custom Development Devices Disease Disease Pathway Doctor of Philosophy Drug Delivery Systems Educational workshop Elements Facility Designs Faculty Fluorescent Probes Genes Genetic Genomics Goals Housing Image Immunoassay Institutes Institution Interdisciplinary Study Investigation Kansas Lung diseases Malignant Neoplasms Maps Mass Spectrum Analysis Mentors Methodology Methods Microfluidics Molecular Molecular Analysis Molecular Probes Monitor Neurosciences Organism Pathologic Processes Pathway interactions Pharmaceutical Chemistry Pharmacologic Substance Phenotype Physiologic Monitoring Process Publications Reagent Research Research Infrastructure Research Personnel Research Project Grants Science Scientist Structure System Technology Universities Zebrafish base chemical genetics chemical synthesis commercialization design empowered genetic manipulation implementation research improved in vivo innovation interest meetings mutant nervous system disorder new technology next generation novel professor programs protein function public health relevance screening symposium therapeutic target tool

项目摘要

DESCRIPTION (provided by applicant): The development of new enabling technologies is critical to improving our understanding and treatment of disease. Immunoassays, gene sequencing, and mass spectrometry represent examples of technologies that have revolutionized biomedical research by allowing the identification of biomarkers and disease pathways that could not have been discovered otherwise. We propose here a new COBRE center focused on Molecular Analysis of Disease Pathways. This Center will bring together junior and senior faculty from the physical, biological, and pharmaceutical sciences at the University of Kansas and other academic institutions in Kansas to conduct multidisciplinary research to develop and implement cutting-edge technologies for elucidating the genetic, chemical, and physical mechanisms of biological processes involved in disease. The scientific emphasis ofthe Center will be to create and implement new enabling technologies for identification of therapeutic targets. These enabling methodologies include methods for integrating next generation gene sequencing with genetic manipulation of model organisms, custom-synthesized fluorescent molecular probes for monitoring physiological/pathological processes in model organisms in vivo, and microfluidic systems for manipulation of model organisms and monitoring of biochemical pathways in vivo. A key innovative element of this proposal is the creation of three core facilities that are designed to operate synergistically: imaging of model organisms treated with fluorescent probes developed by one core facility will be facilitated by the use of microfabricated devices developed in a second core facility; screening of mutant organisms such as C. elegans and zebrafish against these probes will be used to discover novel disease-related phenotypes that can be precisely mapped to identify specific targets through the next generation genomic sequencing and related genomic technologies offered by a third core facility. The proposed Center will exploit the strengths of KU and associated universities in the areas of genetics, bioanalytical chemistry, bioengineering, and chemical synthesis and will create new methodologies and approaches that can be used to investigate any pathway involved in disease. These new technologies will be disseminated through research collaborations, publications, and potential commercialization.

描述（由申请人提供）：新的使能技术的开发对于提高我们对疾病的理解和治疗至关重要。免疫测定、基因测序和质谱分析是通过识别其他方式无法发现的生物标志物和疾病途径而彻底改变生物医学研究的技术的例子。我们在此提议建立一个新的 COBRE 中心，专注于疾病途径的分子分析。该中心将汇集来自堪萨斯大学和堪萨斯州其他学术机构的物理、生物和药物科学领域的初级和高级教师，进行多学科研究，开发和实施阐明遗传、化学和物理的尖端技术。疾病涉及的生物过程的机制。该中心的科学重点将是创建和实施新的使能技术来识别治疗靶点。这些可行的方法包括将下一代基因测序与模式生物的遗传操作相结合的方法、用于监测模式生物体内生理/病理过程的定制合成荧光分子探针、以及用于操作模式生物和监测生物体内生化途径的微流体系统。体内。该提案的一个关键创新要素是创建三个核心设施，这些设施旨在协同运作：通过使用第二个核心设施开发的微型设备，将促进用一个核心设施开发的荧光探针处理的模型生物的成像；针对这些探针对秀丽隐杆线虫和斑马鱼等突变生物进行筛选，将用于发现新的疾病相关表型，通过第三个核心设施提供的下一代基因组测序和相关基因组技术，可以精确绘制这些表型以识别特定目标。拟议的中心将利用 KU 的优势以及遗传学、生物分析化学、生物工程和化学合成领域的相关大学，并将创建可用于研究与疾病有关的任何途径的新方法和途径。这些新技术将通过研究合作、出版物和潜在的商业化来传播。