Biotin Status During Pregnancy

怀孕期间的生物素状态

• 批准号: 8242973
• 负责人: MARIE A CAUDILL
• 金额: $ 7.7万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8242973
• 关键词: Adult; Affect; Animal Model; Biological Markers; Biotin; Blood; Blood specimen; Catabolism; Choline; Chromatin Structure; Cleft Palate; Coenzymes; Data; Development; Diet; Dietary Assessment; Dietary intake; Dose; Energy Metabolism; Ensure; Enzymes; Fetal Development; Fetus; Folate; Food; Gene Expression; Goals; Grant; Health; Histones; Human; Institute of Medicine (U.S.); Intake; Link; Longevity; Lysine; Measurement; Measures; Methodology; Nutrient; Outcome; Participant; Phase; Population; Pregnancy; Pregnant Women; Procedures; Process; Recommended Dietary Allowance; Research; Research Design; Role; Sampling; Sulfoxide; Supplementation; Testing; Therapeutic; Third Pregnancy Trimester; Time; Tissues; Umbilical Cord Blood; United States National Institutes of Health; Urine; Vitamin B Complex; Vitamins; Water; Woman; base; beta-hydroxyisovaleric acid; bisnorbiotin; design; feeding; fetal; fetal programming; human study; improved; insight; meetings; nutrition; pregnant; prenatal; response; urinary

DESCRIPTION (provided by applicant): Although it is recognized that biotin status is compromised during human pregnancy (particularly during the third trimester), no human dietary studies have been conducted to assess dose-response relationships, maternal-fetal compartmentation, or dietary biotin requirements. Consequently, the Food and Nutrition Board of the Institute of Medicine were not able to establish a recommended dietary allowance (RDA) for biotin. Instead an adequate intake (AI) level of 30 mcg biotin per day for adults was estimated without upward adjustments for pregnant women. Goal: The goal of this study is to provide data that will scientifically inform the development of a biotin RDA for pregnant women Specific Aims: The specific aims of this proposal are to: i) test the hypothesis that dietary biotin requirements are increased during pregnancy; ii) test the hypothesis that maternal and fetal biomarkers of biotin status are positively correlated; and iii) investigate the dose-response relationship between dietary biotin intake and validated biomarkers of biotin status. Procedure: To accomplish these aims, a 12-week controlled feeding study conducted in pregnant women (n=10, 21-40y, 27 wk) and nonpregnant control women (n=10, 21-40 y) will be extended to enable a dose response assessment of biotin in these women. Quantitation of the biotin content of the diet will be performed along with measurements of maternal/fetal biomarkers of biotin status including those linked to biotin deficiency (i.e. 3-hydroxyisovaleric acid) and biotin catabolism (i.e. bisnorbiotin and biotin sulfoxide). Expected Outcomes: This study will be able to delineate the relationship between maternal and fetal biotin status in humans; provide insight into the factors that may contribute to the suboptimal biotin status observed during pregnancy; and facilitate an estimation of the level of maternal dietary biotin intake required to meet the increased demands during pregnancy and ensure an adequate supply of biotin to the developing fetus. Collectively, these data are expected to scientifically inform the development of a biotin RDA for pregnant women that is designed to improve maternal and fetal health. PUBLIC HEALTH RELEVANCE: The proposed human study will examine the response of biotin status indicators to a known intake of dietary biotin, assess the distribution of biotin between maternal and fetal compartments, and investigate the relationship between maternal and fetal indicators of biotin status. The data generated from this study are expected to scientifically inform the development of a biotin recommended dietary allowance for pregnant women and guide decisions on the need for biotin supplementation during gestation.

描述（由申请人提供）：尽管人们认识到生物素状态在人类怀孕期间（特别是在妊娠晚期）受到损害，但尚未进行人类饮食研究来评估剂量反应关系、母胎分隔或饮食生物素需求。因此，医学研究所食品和营养委员会无法制定生物素的推荐膳食摄入量 (RDA)。相反，估计成人每天 30 微克生物素的充足摄入量 (AI) 水平，无需对孕妇进行上调。目标：本研究的目的是提供数据，为孕妇生物素 RDA 的开发提供科学依据。 具体目标：本提案的具体目标是： i) 检验妊娠期间膳食生物素需求量增加的假设； ii) 检验母体和胎儿生物素状态生物标志物呈正相关的假设； iii) 研究膳食生物素摄入量与经过验证的生物素状态生物标志物之间的剂量-反应关系。程序：为了实现这些目标，将延长在孕妇（n=10、21-40 岁、27 周）和非怀孕对照妇女（n=10、21-40 岁）中进行的为期 12 周的控制喂养研究，以实现这些女性中生物素的剂量反应评估。饮食中生物素含量的定量将与母体/胎儿生物素状态生物标志物的测量一起进行，包括与生物素缺乏（即3-羟基异戊酸）和生物素分解代谢（即双降生物素和生物素亚砜）相关的标志物。预期结果：这项研究将能够描绘人类母体和胎儿生物素状态之间的关系；深入了解可能导致怀孕期间观察到的生物素状态不佳的因素；有助于估计母亲膳食生物素摄入量，以满足怀孕期间增加的需求，并确保发育中的胎儿有充足的生物素供应。总的来说，这些数据有望为孕妇生物素 RDA 的开发提供科学信息，该生物素 RDA 旨在改善孕产妇和胎儿的健康。 公共健康相关性：拟议的人体研究将检查生物素状态指标对已知膳食生物素摄入量的反应，评估母体和胎儿区室之间生物素的分布，并研究母体和胎儿生物素状态指标之间的关系。这项研究产生的数据预计将为制定孕妇生物素推荐膳食摄入量提供科学依据，并指导妊娠期间是否需要补充生物素的决定。