Vitamin D metabolism in pregnant women consuming controlled intakes

控制摄入量的孕妇的维生素 D 代谢

• 批准号: 9018051
• 负责人: MARIE A CAUDILL
• 金额: $ 7.97万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9018051
• 关键词: 25-hydroxyvitamin D; Address; Alkaline Phosphatase; Animals; Binding; Binding Proteins; Biochemical Markers; Biological; Biological Markers; Blood; Blood Circulation; CYP27B1 gene; Calcium; Cell Culture Techniques; Cells; Child; Collagen; Collagen Type I; Data; Development; Diet; Dietary intake; Dihydroxycholecalciferols; Fetus; Gene Expression; Goals; Health; Human; Incidence; Institute of Medicine (U.S.); Intake; LDL-Receptor Related Protein 2; Link; Maintenance; Measures; Metabolic; Metabolic Pathway; Metabolism; Mixed Function Oxygenases; Modeling; Mothers; Mus; N-telopeptide; Nutrient; Osteocalcin; Outcome; PTH gene; Phosphorus; Placenta; Play; Population; Pregnancy; Pregnant Women; Recommendation; Reporting; Risk; Role; Sampling; Serum; Source; System; Testing; Tissues; Umbilical Cord Blood; Vitamin D; Vitamin D Deficiency; Vitamin D-Binding Protein; Vitamin D2; Woman; Work; base; bone; bone health; bone metabolism; crosslink; deoxypyridinoline; dietary requirement; feeding; fetal; human data; intrinsic factor-cobalamin receptor; maternal serum; pregnant; protein expression; receptor; reproductive; research study

 DESCRIPTION (provided by applicant): Despite growing evidence linking vitamin D to maternal and fetal health outcomes during pregnancy, little is known about the effect of pregnancy on vitamin D metabolism. Notably, the placenta is emerging as a possible source and regulator of circulating maternal vitamin D metabolites because it expresses all components of the vitamin D metabolic pathway and can produce 1,25[OH]2D which doubles during this reproductive state. Nonetheless, few studies have investigated the modulatory role of the placenta on maternal circulating vitamin D metabolites. In addition, although vitamin D is known to influence bone health in the nonpregnant state, it is unclear whether maternal vitamin D status influences maternal and fetal bone health during pregnancy. Animal studies have shown normal bone health outcomes in fetuses from severely vitamin D deficient mice while data from human studies are mixed possibly due to differences among studies in the dietary intakes of calcium, phosphorous, and other nutrients that impact bone health. This study seeks to advance understanding of vitamin D metabolism and requirements during pregnancy by measuring a comprehensive panel of vitamin D biomarkers from pregnant and nonpregnant control women who participated in a feeding study and consumed equivalent intakes of vitamin D and other related nutrients. The Specific Aims are as follows: Aim 1 will test the hypothesis that pregnancy alters blood biomarkers of vitamin D metabolism including 25(OH)D,1,25(OH)2D, 24,25(OH)2D and vitamin D binding protein (DBP). Aim 2 will test the hypothesis that placenta metabolizes vitamin D and contributes to the changes in maternal circulating vitamin D metabolites including 1,25(OH)2D. To achieve this aim, we will measure placental gene and protein expression levels of the megalin-cubilin receptor, 25-hydroxylase (CYP2R1), 1-hydroxylase (CYP27B1), and 24-hydroxylase (CYP24A1) as well as placental metabolite concentrations of 25(OH)D, 1,25(OH)2D, 24,25(OH)2D, and DBP. Relationships between placental and maternal biomarkers of vitamin D will be examined and a human placental cell culture model will be employed to investigate vitamin D metabolic flux and guide interpretation of the placental tissue experiments. Aim 3 will test the hypothesis that maternal serum 25(OH)D levels associate with maternal and fetal markers of bone metabolism. To achieve this aim, we will examine correlations between maternal markers of vitamin D status (i.e., 25[OH]D and 1,25[OH]2D) and maternal/cord blood levels of biochemical markers of bone health (e.g., parathyroid hormone).

 描述（由申请人证明）：尽管有证据表明维生素D在妊娠对维生素D代谢的影响期间，胎盘逐渐成为循环的孕妇的可能来源和招募者。维生素D代谢途径的组成部分可以产生1,25 [OH]的覆盖态，但很少有研究研究了胎盘在母体循环的维生素D代谢物中的调节作用。怀孕期间的非怀孕状态和胎儿骨骼健康表明，由于钙T的饮食量差异，研究骨骼健康的差异可能会使胎儿的胎儿正常通过测量参与兴高采烈营养素的孕妇和非怀孕的妇女的综合维生素D生物标志物，以提高对维生素D代谢和怀孕期间的需求。 25（OH）D，1,25（OH）2D，24,25（OH）2D和维生素D结合蛋白（DBP），将测试维生素TES的假设（OH）2d。 ，将检查2D（OH）2D和DBP。母体血清25（OH）D水平与骨骼代谢的母体和胎儿标记相关。骨骼健康标记（例如甲状旁腺激素）。

项目成果

Maternal vitamin D biomarkers are associated with maternal and fetal bone turnover among pregnant women consuming controlled amounts of vitamin D, calcium, and phosphorus.

• DOI: 10.1016/j.bone.2016.12.002
• 发表时间: 2017-02
• 期刊: Bone
• 影响因子: 4.1
• 作者: Park H;Brannon PM;West AA;Yan J;Jiang X;Perry CA;Malysheva O;Mehta S;Caudill MA
• 通讯作者: Caudill MA