The transcriptional circuitry controlling biofilm development in Candida albicans

控制白色念珠菌生物膜发育的转录电路

基本信息

批准号：
8197358
负责人：
ALEXANDER D JOHNSON
金额：
$ 30.11万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-12-01 至 2015-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8197358
关键词：
Adherence Animals Antifungal Agents Antimicrobial Resistance Back Behavior Biochemical Biological Assay Blood Candida albicans Candidate Disease Gene Catheters Cell Communication Cell-Cell Adhesion Cells Characteristics Communities Data Development Disease Dissection Drug resistance Ectopic Expression Extracellular Matrix Gastrointestinal tract structure Gene Targeting Genes Genetic Genome Genomics Goals Growth Human Immune Immune system Implant In Vitro Individual Infection Knock-out Knowledge Laser Scanning Confocal Microscopy Lead Libraries Life Maintenance Maps Mechanics Medical Device Microbe Microbial Biofilms Microscopy Modeling Monitor Morbidity - disease rate Nature Patients Physiological Process Production Property Proteins Rattus Resistance Resolution Role Skin Tissue Soft Tissue Infections Source Surface Suspension substance Suspensions Systemic infection Testing Venous Virulent Yeasts base chromatin immunoprecipitation feeding genetic analysis improved in vitro testing in vivo in vivo Model microorganism mortality mutant pathogen prevent programs public health relevance research study transcription factor

项目摘要

DESCRIPTION (provided by applicant): The yeast Candida albicans is a normal resident of the human digestive tract. It is also the most common fungal pathogen of humans, causing both mucosal and systemic infections, particularly in immune compromised patients. This proposal seeks to understand how C. albicans orchestrates the formation of biofilms - resilient, surface- associated, organized groups of cells. Biofilm formation is medically relevant because new C. albicans infections are highly correlated with implanted medical devices, which provide efficient substrates for biofilm formation. Our approach to the study of biofilm formation is through dissection of the transcriptional circuitry that controls this process. Our overarching goal is to understand how the individual target genes of the circuit contribute to the key properties of biofilms, such as drug resistance, cell-cell adhesion, and production of extracellular matrix. PUBLIC HEALTH RELEVANCE: C. albicans is the most prevalent fungal pathogen of humans. It causes superficial infections in normal humans and life threatening, systemic infections in immune compromised individuals. This proposal seeks to understand how C. albicans forms biofilms, communities of cells particularly resistant to mechanical force and antifungal drugs. C. albicans biofilms formed on implanted medical devices and are a major source of new infections. Understanding biofilms in more detail may lead to improvements in preventing and treating C. albicans infections.

描述（由申请人提供）：白色念珠菌是人类消化道的普通居民。它也是人类最常见的真菌病原体，引起粘膜和全身感染，特别是在免疫受损的患者中。该提案试图了解白色念珠菌如何策划生物膜的形成 - 弹性，表面相关，有组织的细胞组。生物膜的形成在医学上是相关的，因为新的白色念珠菌感染与植入的医疗设备高度相关，这为生物膜形成提供了有效的底物。我们研究生物膜形成的方法是通过解剖控制这一过程的转录电路。我们的总体目标是了解电路的单个靶基因如何促进生物膜的关键特性，例如耐药性，细胞 - 细胞粘附以及细胞外基质的产生。公共卫生相关性：白色念珠菌是人类最普遍的真菌病原体。它会引起正常人的表面感染和威胁生命，免疫受损的人的全身感染。该提案试图了解白色念珠菌如何形成生物膜，细胞社区，特别是对机械力和抗真菌药物的耐药性。白色念珠菌生物膜在植入的医疗设备上形成，是新感染的主要来源。更详细地了解生物膜可能会导致预防和治疗白色念珠菌感染的改善。