Cationic Amino Acid Transport in Mosquitoes

蚊子中的阳离子氨基酸运输

• 批准号: 8245717
• 负责人: Immo Alex Hansen
• 金额: $ 14.41万
• 依托单位: NEW MEXICO STATE UNIVERSITY LAS CRUCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8245717
• 关键词: Address; Adult; Aedes; Affect; Amino Acid Transporter; Amino Acids; Applications Grants; Arginine; Basic Amino Acid Transport Systems; Biology; Blood; California; Carrier Proteins; Cell membrane; Cloning; Complementary DNA; Culicidae; Dengue; Development; Family; Fat Body; Female; Fertility; Foundations; Funding; Gene Expression Profile; Goals; Health; Histidine; Housing; Human; Immune system; Immunity; Injection of therapeutic agent; Insecta; Investigation; Kinetics; Knowledge; Laboratories; Length; Life Style; Longevity; Lysine; Malaria; Mediating; Membrane Transport Proteins; Mentors; Messenger RNA; Molecular; Nutritional; Nutritional status; Organ Culture Techniques; Patch-Clamp Techniques; Physiological; Physiology; Predisposition; Property; Proteins; Publications; RNA Interference; Reproduction; Research; Research Personnel; Role; Signal Transduction; Spliced Genes; Substrate Specificity; System; Techniques; Testing; Tissues; Universities; Variant; Vertebrates; Work; Xenopus; Xenopus oocyte; Yellow Fever; abstracting; expression vector; positional cloning; post-doctoral training; preference; public health relevance; solute; uptake; vector; vector mosquito

DESCRIPTION (provided by applicant): Project Summary/Abstract The main goal of the proposed study is the molecular characterization of the cationic amino acid (AA) translocation system in the fat body of the Yellow fever mosquito Aedes aegypti, the principle vector for Dengue fever. As an adaption to their unique life style, the fat body tissue of adult mosquitoes houses an AA sensing and transport system which is essential for reproduction. The nutritional status of mosquitoes affects this system. The transport of cationic AAs over the fat body plasma membrane after a blood meal is indispensable for mosquito reproduction. In vertebrates cationic AAs are translocated by proteins of the Solute Carrier Family 7 (SLC7). We have identified one transporter of the SLC7 family as a transporter for the cationic AA L-histidine in mosquitoes; however, there has been no comprehensive investigation into the transport mechanisms of the other cationic AAs so far. Consequently, cationic AA transporters, their substrate specificities, and transport kinetics have not been identified. To address this problem we will use a combination of pyrosequencing, reverse genetics, and electrophysiological techniques. The proposed work will achieve a significant gain of knowledge in the field of AA transport in vector mosquitoes. . PUBLIC HEALTH RELEVANCE: Project Narrative The proposed work will prove significant for human health through the identification and characterization of essential and mosquito/insect-specific transporters that present high-value targets for the development of insecticidal substances.

描述（由申请人提供）：拟议研究的项目摘要/摘要是在黄热蚊子埃德斯伊斯特（Aeedes aegypti）脂肪体中阳离子氨基酸（AA）易位系统的分子表征，这是登革热的原理矢量。为了适应其独特的生活方式，成年蚊子的脂肪身体组织容纳了AA感应和运输系统，这对于繁殖至关重要。蚊子的营养状况会影响该系统。血粉后，阳离子AAS在脂肪体质膜上的运输对于蚊子繁殖是必不可少的。在脊椎动物中，阳离子AAS由溶质载体家族7（SLC7）的蛋白质易位。我们已经将SLC7家族的一个转运蛋白确定为蚊子中阳离子AA L-杀菌素的转运蛋白。但是，到目前为止，还没有对其他阳离子AAS的运输机制进行全面调查。因此，尚未确定阳离子AA转运蛋白，其底物特异性和运输动力学。为了解决这个问题，我们将使用焦磷酸测序，反向遗传学和电生理技术的组合。拟议的工作将在媒介蚊子中AA运输领域获得大量知识。 。 公共卫生相关性：项目叙述拟议的工作将通过鉴定和表征基本和蚊子/昆虫特异性转运蛋白来证明对人类健康的重要性，这些转运蛋白具有高价值靶标，用于开发杀虫物质。

项目成果

Four-way regulation of mosquito yolk protein precursor genes by juvenile hormone-, ecdysone-, nutrient-, and insulin-like peptide signaling pathways.

• DOI: 10.3389/fphys.2014.00103
• 发表时间: 2014
• 期刊: Frontiers in physiology
• 影响因子: 4
• 作者: Hansen IA;Attardo GM;Rodriguez SD;Drake LL
• 通讯作者: Drake LL

The fat body transcriptomes of the yellow fever mosquito Aedes aegypti, pre- and post- blood meal.

• DOI: 10.1371/journal.pone.0022573
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Price DP;Nagarajan V;Churbanov A;Houde P;Milligan B;Drake LL;Gustafson JE;Hansen IA
• 通讯作者: Hansen IA

SLC7 amino acid transporters of the yellow fever mosquito Aedes aegypti and their role in fat body TOR signaling and reproduction.

• DOI: 10.1016/j.jinsphys.2012.01.005
• 发表时间: 2012-04
• 期刊: Journal of insect physiology
• 影响因子: 2.2
• 作者: Carpenter VK;Drake LL;Aguirre SE;Price DP;Rodriguez SD;Hansen IA
• 通讯作者: Hansen IA