Characterization of amino acid transport- and signaling in the mosquito fat body

蚊子脂肪体中氨基酸转运和信号传导的表征

基本信息

批准号：
8474239
负责人：
Immo Alex Hansen
金额：
$ 36.5万
依托单位：
NEW MEXICO STATE UNIVERSITY LAS CRUCES
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-09 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8474239
关键词：
Address Aedes Amino Acid Transporter Amino Acids Arginine Basic Amino Acid Transport Systems Biochemistry Biological Assay Biological Models Biology Carrier Proteins Chemicals Communities Complement Computer Simulation Country Culicidae Data Set Dengue Development Disease Vectors Eligibility Determination Essential Amino Acids Family Fat Body Generations Genes Goals Health Histidine Human Immunity In Vitro Insecta Insecticides Kinetics Knowledge Literature Lysine Mediating Metabolic Pathway Metabolism Molecular Biology Mutagenesis Mutation Nutrient Organic Chemistry Pathway interactions Phenotype Proteins Public Health RNA Interference Reproduction Research Role Signal Pathway Signal Transduction Specificity Structure Substrate Specificity System Testing Time Work Yellow Fever analog arbovirus disease base design extracellular fitness improved in vivo inhibitor/antagonist interdisciplinary approach metabolomics novel public health relevance three-dimensional modeling transcriptomics transport inhibitor uptake vector vector control

项目摘要

DESCRIPTION (provided by applicant): New insecticides with novel modes of action are urgently needed to improve public health in many countries around the world. This project addresses several important questions regarding nutrient transport and signaling pathways in the fat body of the yellow fever mosquito, Aedes aegypti. We will characterize the mode of action of two essential mosquito cationic amino acid transporters and test and improve promising compounds to inhibit cationic amino acid transport. We will complement these approaches with the development of an in silico mosquito fat body gene knowledge network designed to identify genes in the amino acid signaling and transport cascades downstream of cationic amino acid transporters. The proposed work will prove significant for human health through the significant gain of knowledge on amino acid transport and signaling in mosquitoes. Amino acid transport inhibitors have the potential to give rise to a new generation of public health insecticides. In addition, this study will expand our knowledge of the functional mechanism of cationic amino acid transporter in insects with implications for understanding essential amino acid transporters in humans and other model systems.

描述（由申请人提供）：迫切需要采用新型作用方式的新杀虫剂来改善世界上许多国家的公共卫生。该项目解决了有关养分运输和信号通路的几个重要问题，该蚊子的脂肪体是埃及埃及埃及的脂肪体。我们将表征两个必需蚊子氨基酸转运蛋白的作用方式，并测试并改善有希望的化合物以抑制阳离子氨基酸的转运。我们将通过开发硅酸信号传导中的基因和在阳离子氨基酸转运蛋白下游的级联反应中鉴定基因的基因知识网络的开发来补充这些方法。拟议的工作将通过对蚊子中的氨基酸传输和信号传导的大量知识的大量获取对人类健康产生重要意义。氨基酸转运抑制剂有可能引起新一代的公共卫生杀虫剂。此外，这项研究将扩大我们对昆虫阳离子氨基酸转运蛋白功能机理的了解，对了解人类和其他模型系统中必需的氨基酸转运蛋白的影响。