NMR Metabolomics Core/SNYDER

NMR 代谢组学核心/SNYDER

• 批准号: 8534960
• 负责人: SUSAN J SUMNER
• 金额: $ 23.36万
• 依托单位: RESEARCH TRIANGLE INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-04 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8534960
• 关键词: Adopted; Age; Biochemical Pathway; Biochemistry; Biological; Biological Markers; Biomedical Research; Brain; Cell Extracts; Cells; Client; Code; Collaborations; Complement; Consult; Consultations; Contracts; Data; Data Analyses; Data Files; Databases; Deposition; Detection; Discriminant Analysis; Disease; Dose; Ensure; Ethnic Origin; Functional disorder; Funding; Gender; Generations; Grant; Health; Housing; Human Subject Research; Instruction; Internships; Intervention; Investigation; Kidney; Least-Squares Analysis; Libraries; Link; Liquid substance; Liver; Mann-Whitney U Test; Maps; Mass Spectrum Analysis; Measures; Methods; Molecular Weight; NMR Spectroscopy; Nuclear Magnetic Resonance; Online Systems; Outcome; Pathway interactions; Pattern; Phenotype; Plasma; Preparation; Principal Component Analysis; Process; Publications; Quality Control; Race; Reproducibility; Research Design; Research Institute; Research Personnel; Resources; Rodent Model; Sampling; Scientist; Serum; Signal Transduction; Stream; Structure; Testing; Testis; Tissue Extracts; Tissues; Training; Urine; Uterus; Work; base; cost; cost effective; data sharing; experience; insight; meetings; member; metabolomics; novel strategies; nutrition; outreach; programs; response

The NMR Metabolomics Core will be responsible for the generation and analysis of metabolomic profiles of biological fluids and tissue extracts. This Core will support the RTI RCMRC by providing standardized analysis and data in a consistent and coherent format, and providing expertise for interpreting results in the context of the study design and other phenotypic measures and data streams. Studies within the RTI Metabolomics Program have included the analysis of samples (e.g., urine, serum, extracts of serum, plasma, liver, kidney, brain, uterus, testes) from dose-response studies in rodent models to provide mechanistic insights and to develop biomarkers that are predictive of adverse health outcomes. The NMR Metabolomics Core also has experience evaluating samples from human subject research aimed at examining the responsivity to treatment and disease endpoints. The NMR Metabolomics Core is supported by our partners from the David H. Murdock Research Institute, which houses state-of-the-art 700 MHz and 950 MHz NMR spectrometers. Three internationally recognized metabolomics scientists (Dr. Jeremy Nicholson, Dr. Elaine Holmes, and Dr. Ian Wilson) will support the RTI RCMRC though consultation and collaboration. This core will ensure that the most cost-effective approach is used by conducting analysis of samples in appropriate size batches to reduce costs. Quality control features will include the use of internal standards, prior determination of reproducibility of methods with repeat measures, using single sample code throughout all aspects of center activities, and appropriate audits. The Core members will work with the RTI RCMRC Management Team to develop consistent formats and acceptable formats to deposit raw data or processed data files to the Data Repository and Coordinating Center according to the data sharing plan and requirements and the EC. The NMR Core will develop and adopt new methods as deemed appropriate by the EC. Members of the NMR Core will work with the Promotion and Outreach Core to develop materials for dissemination at meetings, and to provide web-based and internship training. The NMR Core will support the Mass Spectrometry Cores through metabolite identification.

NMR代谢组学核心将负责生成和分析生物液和组织提取物的代谢组谱。该核心将通过以一致且连贯的格式提供标准化的分析和数据来支持RTI RCMRC，并在研究设计和其他表型措施和数据流的背景下提供专业知识来解释结果。 RTI中的研究 代谢组学计划包括对啮齿动物模型中剂量反应研究的样品分析（例如，尿，血清，血清，血浆，肝脏，肾脏，脑，子宫，子宫，子宫，睾丸的提取物），以提供机械洞察力，以提供机械洞察力并开发出可预测不良健康的生物标志物。 NMR代谢组学核心还具有评估人类学科研究样本的经验，旨在检查 对治疗和疾病终点的反应。 NMR代谢组学核心得到了David H. Murdock研究所的合作伙伴的支持，该研究所拥有最先进的700 MHz和950 MHz NMR光谱仪。三位国际认可的代谢组学科学家（Jeremy Nicholson博士，Elaine Holmes博士和Ian Wilson博士）将通过咨询和协作来支持RTI RCMRC。这个核心 将通过对适当尺寸批次的样品进行分析以降低成本来确保使用最具成本效益的方法。质量控制功能将包括使用内部标准，先前确定具有重复度量的方法的可重复性，在中心活动的所有方面使用单个示例代码以及适当的审核。核心成员将与RTI RCMRC管理团队合作，以开发一致的格式和可接受的格式，以将原始数据或处理的数据文件存放到数据存储库中，并根据数据共享计划和要求以及EC进行协调中心。 NMR核心将开发并采用EC认为适当的新方法。 NMR Core的成员将与促销和外展核心合作，在会议上开发材料，并提供基于网络的实习培训。 NMR核心将通过代谢物鉴定支持质谱核心。