Flow

流动

基本信息

批准号：
7945044
负责人：
Richard G. Vile
金额：
$ 12.94万
依托单位：
MAYO CLINIC ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-17 至 2014-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7945044
关键词：
Air Antibodies Apoptosis Apoptotic Archives Area Arizona Binding Blood Bone Marrow Budgets Calcium Cancer Center Cell Communication Cell Cycle Cell Cycle Regulation Cell Separation Cell Survival Cells Characteristics Clinic Clone Cells Cloning Color Complex Comprehensive Cancer Center Computer software Computers Confocal Microscopy Core Facility Cyan Fluorescent Protein DNA DNA Nucleotidylexotransferase DNA Probes DNA strand break Data Data Analyses Data Files Detection Discrimination Disease Progression Drops Electrophysiology (science)Enzymes Equipment Event Experimental Designs Florida Flow Cytometry Fluorescence Fluorescence Microscopy Fluorescence Recovery After Photobleaching Fluorescence Resonance Energy Transfer Fluorescent Probes Fluorochrome Follow-Up Studies Genetic Grant Green Fluorescent Proteins Heating Housing Human Resources Image Image Analysis Immunology Individual Ions Kinetics Label Laboratories Lasers Learning Life Light Microscope Liquid substance Maintenance Mayo Clinic Cancer Center Measures Mediating Membrane Microinjections Microscope Microscopy Minor Modeling Molecular Medicine Monoclonal Antibodies Morphology Neurons Operative Surgical Procedures Optics Organism Pharmaceutical Preparations Phase Phosphatidylserines Ploidies Population Population Study Procedures Process Propidium Diiodide Protein Analysis Proteins RNA Relative (related person)Research Research Personnel Resolution Resource Sharing Resources Running Sampling Scanning Services Shapes Side Site Sorting - Cell Movement Specialist Specimen Staging Statistical Data Interpretation Stimulus Students Suggestion Surface Suspension substance Suspensions System Techniques Technology Time Tracer Training Transfection Travel Tube Update Writing annexin A5 base cancer therapy culture plates cytokine detector digital digital imaging experience improved instrument instrumentation interest laser cell sorter light microscopy member nanocrystal professor programs ratiometric receptor receptor density reconstruction research study theories tissue culture tool treatment effect web site

项目摘要

The Flow Cytometry/Optical Morphology Shared Resource of the Mayo Clinic Cancer Center (MCCC) provides instrumentation, expertise, and training related to these two areas. Flow cytometry is used for highspeed analysis and sorting of cells in liquid suspension. Optical morphology refers to techniques associated with light microscopy and image analysis. The flow cytometry area houses instruments of varying capabilities from a single laser bench-top analytical system to a five-laser, four-way cell sorter. Investigators, students, and technicians have the option of dropping off prepared samples to be run by resource personnel or to be trained to operate any of the five analytical flow cytometers. Resource personnel perform cell sorting. The optical morphology area houses a variety of light microscopy based instruments including laser scanning confocal microscopes and other upright and inverted light microscopes equipped with specialized components for optical sectioning, microinjection, ratiometric imaging, and total internal reflection fluorescence (TIRF). Training is provided for all of these microscopy techniques. Image analysis software including customized macro programming along with appropriate training is also available. Resource personnel are available to assist with training, troubleshooting, maintenance, data interpretation, and experimental design.

梅奥诊所癌症中心 (MCCC) 的流式细胞术/光学形态学共享资源提供与这两个领域相关的仪器、专业知识和培训。流式细胞术用于高速液体悬浮液中细胞的分析和分选。光学形态学是指相关技术通过光学显微镜和图像分析。流式细胞仪区域配备了不同类型的仪器从单激光台式分析系统到五激光、四路细胞分选仪的功能。调查人员，学生和技术人员可以选择将准备好的样品交给资源人员运行或接受培训以操作五种分析流式细胞仪中的任何一种。资源人员执行单元排序。光学形态区域拥有各种基于光学显微镜的仪器，包括激光扫描共焦显微镜和其他正置和倒置光学显微镜配备了专门的用于光学切片、显微注射、比例成像和全内反射的组件荧光（TIRF）。为所有这些显微镜技术提供培训。图像分析软件还提供定制宏编程以及适当的培训。资源人员可协助培训、故障排除、维护、数据解释和实验设计。