RNASE P

核糖核酸酶P

• 批准号: 8365869
• 负责人: DAVID R ENGELKE
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365869
• 关键词: Affinity; Biology; Funding; Fungal Genome; Grant; Holoenzymes; Lead; National Center for Research Resources; Nuclear; Principal Investigator; Process; Proteins; RNase P; Research; Research Infrastructure; Resources; Role; Source; United States National Institutes of Health; Work; Yeasts; cost; novel; tRNA Precursor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The yeast nuclear RNase P is responsible for the maturation of the 5' end of precursor tRNAs. There are several lines of evidence that lead us to believe that RNase P has additional substrates and we have recently identified several candidiates. Work to attempt the identification of interacting partners outside the known core of RNase P is being carried out. Using novel affinity tags, we are able to rapidly isolate the holoenzyme and expect to find interacting proteins that were lost during the initial arduous purification that led to the identification of the RNase P core. These interacting partners will be investigated for their roles in the processing of novel substrate candidates recently identified in our lab.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 酵母核RNase P负责前体TRNA的5'末端的成熟。有几条证据使我们相信RNase P还有其他底物，我们最近确定了几个候选人。 正在尝试识别在RNase P的已知核心之外的互动伙伴的工作。使用新颖的亲和力标签，我们能够快速分离全酶，并期望在初始艰苦的纯化过程中找到丢失的相互作用蛋白，从而导致RNase P核心鉴定。这些相互作用的伙伴将在我们实验室最近确定的新型底物候选者处理中的作用。