TAP TAG PULL DOWN OF NOVEL PROTEINS INVOLVED IN TGM SILENCING

TAP 标签下拉参与 TGM 沉默的新型蛋白质

• 批准号: 7420796
• 负责人: DAVID R ENGELKE
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420796
• 关键词: DNA directed RNA polymerase; genes; proteins

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There are many types of regulatory elements at the transcriptional level. Some types of regulatory elements cause positive enhancement of transcription and other negative regulation or ¿¿silencing.¿¿ In Saccaromyces cerevisiae some of the most studied and understood types are silencing near telomeres and silent mating loci. Our lab has identified a novel form of silencing in yeast which involves the transcription of tRNA genes by RNA polymerase III. The transcription of pol III tRNA genes has been shown to cause the inhibition of certain RNA polymerase II genes. Deletion of genes shown to cause alleviation of other types of silencing has shown to have no affect on TGM silencing, however previous studies in our lab has uncovered novel proteins that alleviates this phenomena. However, none of these proteins has been shown to possess any silencing ability or to interact with each other. It is our belief that one of these proteins must be interacting with proteins that directly lead to silencing that the tRNA loci. We have acquired duel affinity versions of these proteins and will do pull downs and then use Mass spectrometry to identify novel protein interactions.

该主题是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是针对该中心的，这不是调查人员的机构。转录级别有许多类型的调节元素。某些类型的调节元件会导致转录和其他负调节的正面增强或沉默。我们的实验室已经确定了酵母中一种新型的沉默形式，涉及RNA聚合酶III的TRNA基因的转录。 POL III tRNA基因的转录已被证明会导致某些RNA聚合酶II基因的抑制。证明会导致其他类型的沉默的基因的缺失证明对TGM沉默没有影响，但是我们实验室中的先前研究发现了减轻这种现象的新型蛋白质。但是，这些蛋白质都没有证明具有任何沉默能力或相互作用。我们相信，这些蛋白质之一必须与直接导致TRNA基因座沉默的蛋白质相互作用。我们已经获得了这些蛋白质的适当亲和力版本，并将进行下降，然后使用质谱法来识别新型的蛋白质相互作用。