SRLS APPROACH FOR STUDIES ON ORIENTED MEMBRANE PROTEINS BY SOLID-STATE NMR

通过固态核磁共振研究定向膜蛋白的 SRLS 方法

• 批准号: 8364106
• 负责人: Alexander A. Nevzorov
• 金额: $ 0.16万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364106
• 关键词: Accounting; Address; Data; Diffusion; Evolution; Funding; Grant; Length; Magnetism; Membrane; Membrane Proteins; Methods; Modeling; Motion; NMR Spectroscopy; National Center for Research Resources; Phase; Physiologic pulse; Principal Investigator; Protein Dynamics; Proteins; Relaxation; Research; Research Infrastructure; Resources; Source; Technology; Time; United States National Institutes of Health; Vertebral column; cost; research study; solid state nuclear magnetic resonance

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In solid-state NMR of oriented membrane proteins, the traditional method of NMR spin relaxation analysis, which assumes predominantly fast isotropic motions, does not extract adequately and fully the information inherent to the experimental data. Here, the primary issue is how to address the great complexity of protein dynamics, including the global and restricted local motions. In general, in oriented membrane proteins one should expect highly constrained local motions of the loop domains and the backbone superimposed onto the overall uniaxial rotational diffusion of the protein (e.g. of the type observed in magnetically aligned bicelles. The unique SRLS approach developed by Freed and co-workers accounts most generally for the relationship between the global and local motions, for the local ordering, and for the relevant magnetic interactions. The implementation of the model for the 2D-ELDOR experiment is directly generalizable to solid-state NMR pulse sequences, which include switching on the transverse B1 field with defined pulse phases and lengths for prolonged periods of time during the evolution. Moreover, the features of the SRLS model allow one to include torsional motions of the protein within the membrane's restoring potential, as well as the effects of collective fluctuations of the membrane on the protein dynamics. We will adapt the SRLS model to solid-state NMR spectroscopy of oriented membrane proteins with the purpose of extracting the dynamic and structural information from the analyses of spectral lineshapes and cross-polarization build-up rates.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在定向膜蛋白的固态NMR中，传统的NMR自旋弛豫分析方法主要假定快速快速的各向同性运动，并不能充分且充分提取实验数据固有的信息。在这里，主要问题是如何解决蛋白质动力学的重大复杂性，包括全球和受限的局部动作。 通常，在定向的膜蛋白中，一个人应该期望循环域的高度约束局部运动，而骨架叠加到蛋白质的整体单轴旋转扩散层（例如，在磁性偏好的bicelles中观察到的类型的类型。 2D-ELDOR实验的模型可以直接推广到固态NMR脉冲序列，其中包括在横向B1场上切换定义的脉冲阶段和长度的长度，并且在演变过程中，SRLS的特征允许SRLS的特征在于弹性的范围内，并在杂项中包括了对质量效果的效果。蛋白质动力学。