POTASSIUM CHANNEL SELECTIVITY FILTER

钾通道选择性过滤器

• 批准号: 8361639
• 负责人: Eduardo A Perozo
• 金额: $ 1.65万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361639
• 关键词: Event; Funding; Goals; Grant; Molecular; Molecular Analysis; Molecular Conformation; Mutation; National Center for Research Resources; Potassium Channel; Principal Investigator; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; cost; design; mutant; prokaryotic potassium channel; sensor; structural biology; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The long-term goal of this project is to further understand the molecular mechanisms of gating in voltage-dependent channels, by focusing on the analysis of K+ channel gating in KcsA and KvAP. Analysis of the molecular identity of the pH sensor in the prokaryotic potassium channel KcsA identified several key mutants that stabilized the KcsA inner bundle gate in the fully open conformation at neutral pH, a fact that makes possible the crystallographic analyses of KcsA in a variety of functional states. Additional mutations in the selectivity filter were designed towards the determination of a fully conductive and an inactivated form of the channel and the probing of modal gating events.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目的长期目标是通过关注KCSA和KVAP中K+通道门控的分析，进一步了解电压依赖性通道中门控机制的分子机制。对原核钾通道KCSA中pH传感器的分子身份的分析确定了几个关键突变体，这些突变体稳定了中性pH的完全开放构型中的KCSA内束门，这一事实使KCSA在多种功能状态中的晶体学分析成为可能。选择性滤波器中的其他突变是为了确定通道的完全导电和灭活形式以及模态门控事件的探测。