HIGH SCHOOL MARINE PHAGE PROJECT

高中海洋噬菌体项目

• 批准号: 8362454
• 负责人: CLINTON S POTTER
• 金额: $ 0.64万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362454
• 关键词: Bacteriophages; Carbon; Film; Funding; Grant; Image; Marines; Microscopy; Molecular; National Center for Research Resources; Preparation; Principal Investigator; Protocols documentation; Reporting; Research; Research Infrastructure; Resources; Sampling; Seawater; Site; Source; Students; United States National Institutes of Health; Work; Writing; cost; high school; programs; water sampling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. As part of an 8 week summer intern program for high school students, we developed a research program where the intern collects sea water samples from several sites and then analyzes TEM images for the presence of phages. The program has been developed so that the intern is semi-independent in determining the research plan. A simple protocol is followed for sample preparation that centrifuges filtered sea water onto a grid with a carbon film. The grids are then imaged using Leginon and the TEM and the images are then analyzed for the presence of phages. The intern writes a report and gives a presentation. The intern works closely with NRAMM staff and by the end of the summer becomes familiar with the scientific research envirnonment.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 作为一个为高中生的为期8周的暑期实习计划的一部分，我们开发了一个 实习生从多个站点收集海水样本，然后 分析TEM图像是否存在噬菌体。该程序已经开发 实习生在确定研究计划方面是半独立的。 一个简单的协议是 接下来进行样品制备，离心将海水过滤到带有碳的网格上 电影。然后使用Leginon对网格进行成像，然后TEM和图像是 分析了存在噬菌体的存在。 实习生写了一份报告并提供了演讲。 实习生与NRAMM员工紧密合作，到夏季结束时 与科学研究环境一起。