High Throughput Data Collection Software for Multiplexed TEM Samples

适用于多重 TEM 样品的高通量数据采集软件

• 批准号: 8712794
• 负责人: CLINTON S POTTER
• 金额: $ 22.32万
• 依托单位: NANOIMAGING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-20 至 2015-06-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8712794
• 关键词: Address; Adoption; Antibodies; Architecture; Automation; Behavior; Benchmarking; Biodistribution; Biological; Biological Models; Complement; Computer software; Data; Data Collection; Data Set; Detection; Development; Documentation; Drug Delivery Systems; Ensure; Environment; Evaluation; Future; Generations; Goals; Health; Hour; Image; Link; Location; Measurement; Methods; Microscope; Monitor; Nanotechnology; Online Systems; Performance; Pharmacologic Substance; Phase; Process; Property; Research Personnel; Resolution; Running; Safety; Sampling; Shapes; Specific qualifier value; Specimen; Stress; System; Techniques; Technology; Testing; Therapeutic; Time; Toxic effect; Transmission Electron Microscopy; Vaccines; Virus; base; biophysical techniques; cost; data acquisition; design; image processing; improved; nanoparticle; nanorod; physical property; predictive modeling; prototype; public health relevance; research and development; screening; software development; therapeutic vaccine; three dimensional structure; tool

DESCRIPTION (provided by applicant): Transmission electron microscopy is a valuable technique for characterizing biological therapeutics, vaccines, drug delivery vehicles, and nanoparticles. There is considerable evidence that links the physical properties of these samples to their bio-distribution, safety, and efficacy. TEM imaging allows direct observation and quantification of these parameters, which include size distributions, shape distributions, concentrations, aggregation states, and 2D and 3D structure determinations. This wealth of information is especially useful when researchers must troubleshoot problems of unknown origin. As bio-therapeutics and nanoparticles increase in size and complexity, TEM is becoming increasingly important as an orthogonal complement to other biophysical methods. Despite the obvious utility of TEM as a direct technique for describing the physicochemical properties of samples in a variety of relevant biological environments, the cost per sample is a significant barrier to the method's widespread adoption, particularly for routine screening of samples. To address this, we will develop software focused on high-throughput data acquisition of multiple samples applied to a single grid substrate. Our long-term goal is to lower the cost per sample for TEM by at least an order of magnitude, and thus establish TEM as an affordable and ubiquitous technique for commercial R&D and testing.

描述（由申请人提供）：传输电子显微镜是一种表征生物学治疗，疫苗，药物输送车和纳米颗粒的宝贵技术。有大量证据将这些样品的物理特性与它们的生物分布，安全性和功效联系起来。 TEM成像允许直接观察和量化这些参数，其中包括大小分布，形状分布，浓度，聚合状态以及2D和3D结构的确定。当研究人员必须对未知来源的问题进行故障排除时，这些信息特别有用。随着生物治疗和纳米颗粒的大小和复杂性的增加，作为对其他生物物理方法的正交补充，TEM变得越来越重要。尽管TEM是一种直接技术，可以在各种相关的生物环境中描述样品的物理化学特性，但每个样品的成本是该方法广泛采用的重要障碍，尤其是对于样品的常规筛选。为了解决这个问题，我们将开发针对应用于单个网格基板的多个样本的高通量数据采集的软件。我们的长期目标是将TEM的每个样本成本降低至少一个数量级，从而将TEM确立为一种负担得起的无处不在的技术，用于商业研发和测试。