CORTICAL AND HIPPOCAMPAL MORPHOMETRY IN A FAMILY WITH MOOD DISORDERS:

情绪障碍家庭的皮质和海马形态测量：

• 批准号: 8363440
• 负责人: MARINA BOCCARDI
• 金额: $ 1.01万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363440
• 关键词: Affect; Algorithms; Anatomy; Atrophic; Cerebrum; Characteristics; Computer software; Corticosteroid Receptors; Data; Disease; Emotional; Family; Frequencies; Funding; Grant; Hippocampal Formation; Hippocampus (Brain); Image; Individual; Lateral; Location; Magnetic Resonance Imaging; Maps; Measurement; Measures; Medial; Mental Depression; Methods; Modeling; Mood Disorders; Morphology; National Center for Research Resources; Parahippocampal Gyrus; Patients; Pattern; Perception; Principal Investigator; Process; Protocols documentation; Radial; Relative (related person); Research; Research Infrastructure; Resources; Severities; Slice; Source; Structure; Surface; Techniques; Testing; Thick; Tissues; United States National Institutes of Health; brain morphology; computational anatomy; cost; density; frontal lobe; gray matter; hippocampal morphometry; hippocampal subregions; indexing; white matter

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A CPM and Radial Atrophy Mapping Background: little information is available on the cerebral characteristics of familial mood disorders. Medial temporal abnormalities have been described in such patients, but data are not consistently replicated, nor a univocal pathogenetic explanation of findings has been provided. Fine techniques like the cortical pattern matching (CPM) or the radial atrophy mapping are able to detect in great detail the morphology of the cortical gyri and the hippocampus, and might provide a detailed characterization of individuals coming from families carrying this kind of disease. Methods: MRI images from related subjects affected and non affected by mood disorders, and from matched healthy controls have been collected with 1.0 Tesla Philips Gyroscan (PG) in Brescia. Images were acquired with gradient echo 3D technique as follows: TR = 20 ms, TE = 5.0 ms, flip angle = 30¿, field of view = 220 mm, acquisition matrix 256x256, slice thickness 1.3 mm. MRI images will be processed with two kinds of analysis: the cortical pattern matching technique and the hippocampal radial atrophy mapping. CPM: the algorithm will be used used to identify regions where the cortical gray matter density will be different in cases vs controls. MR images will be normalized to a customized template using a 12 parameter linear transformation and 3D cortical surfaces of both hemispheres will be extracted; 29 sulci will be manually outlined on the lateral and medial surface of each hemisphere, and additional 3D lines will be drawn to delimit interhemispheric gyral limits. A customized template will be created averaging the traced sulci of the analyzed subjects, and the individual sulci will be used as landmarks to warp each subject's anatomy to the template. Original MR images will be segmented into gray matter, white matter, and CSF, and the warping fields obtained with cortical pattern matching will be applied to the GM images, thus allowing measurement of GM at thousands of homologous cortical locations. The mean gray matter proportion will be computed and a statistical significance map will be created using a t-test to compare affected patients vs non related controls, non affected relatives vs controls and affected patients vs non affected relatives. Hippocampal radial mapping: MRI images will be normalized by linear (12 parameter) transformation to a customized template using the Statistical Parametric Mapping (SPM99) software. The hippocampi will be manually traced according to a formal protocol with established inter- and intra-rater reliability and 3D parametric surface mesh models will be created to represent the hippocampus in each subject. To assess hippocampal morphology, a medial curve will be automatically defined as the 3D curve traced out by the centroid of the hippocampal boundary in each image slice. The radial size of each hippocampus at each boundary point will be assessed by automatically measuring the radial 3D distance from the surface points to the medial curve defined for individual's hippocampal surface model. Shorter radial distances will be used as an index of atrophy. Statistical maps will be generated indicating local group differences in radial hippocampal distance. Expected results: altered brain morphology, likely consisting in reduced volumes, is expected in the frontal lobes and in other structures, like the insular region, devoted to proper perception and control of emotional states. Altered morphology of the hippocampal formation is also expected, mainly in subjects with greater severity or frequency of depression episodes. Greater tissue reduction in the hippocampal subregions with greater concentration of corticosteroids receptors is expected.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 CPM和径向萎缩映射 背景：关于家庭情绪障碍的大脑特征的信息很少。在此类患者中已经描述了媒体暂时的异常，但是数据并未始终如一地复制，也没有提供对发现的单次致病解释。诸如皮质模式匹配（CPM）或径向萎缩映射之类的精细技术能够详细检测皮质回和海马的形态，并可能提供来自携带这种疾病家庭的人的详细表征。 方法：来自布雷西亚的1.0特斯拉飞利浦陀螺仪（PG）收集了受情绪障碍影响和不影响情绪障碍的相关受试者的MRI图像。使用梯度回声3D技术获取图像如下：TR = 20 ms，TE = 5.0 ms，Flip Angle = 30讲，视场= 220 mm，Acquisition Matrix 256x256，切片厚度1.3 mm。 MRI图像将通过两种分析处理：皮质模式匹配技术和海马径向萎缩映射。 CPM：该算法将用于识别在情况与对照中皮质灰质密度不同的区域。 MR图像将使用12个参数线性变换标准化为定制的模板，并且将提取两个半球的3D皮层表面； 29沟将在每个半球的侧面和内侧表面手动概述，并将绘制其他3D线以界定半球间的循环极限。将创建一个定制的模板，平均分析受试者的追溯沟，并且单个沟将用作地标，以将每个受试者的解剖结构与模板扭曲。原始的MR图像将分为灰质，白质和CSF，并将使用皮质图案匹配获得的翘曲场应用于GM图像，从而可以在数千个同源皮质位置测量GM。将计算平均灰质比例，并将使用t检验创建统计显着性图，以比较受影响的患者与非相关对照，非受影响的亲戚与对照对照，并且受影响的患者与非受影响的亲戚。 海马径向映射：MRI图像将通过使用统计参数映射（SPM99）软件进行线性（12个参数）转换为自定义模板进行标准化。海马将根据具有建立的评估者间可靠性的形式协议手动追踪，并将创建3D参数表面网格模型以表示每个受试者的海马。为了评估海马形态，将自动将内侧曲线定义为每个图像切片中海马边界的质心所追踪的3D曲线。每个边界点的每个海马的径向大小将通过自动测量从表面点到定义为个人海马表面模型定义的内侧曲线的径向3D距离来评估。较短的径向距离将用作萎缩的指数。将生成统计图，表明径向海马距离的局部组差异。 预期的结果：脑形态的改变，可能由减少体积组成，在额叶爱情和其他结构（例如岛屿区域）中预期，专门用于对情绪状态的适当感知和控制。 预计海马形成的形态改变了，主要是在抑郁发作的严重程度或频率更高的受试者中。期望具有更大浓度的皮质类固醇受体的海马子区域的组织减少。