ELECTRONIC NOTEBOOK FOR CRYO-EM

用于冷冻电镜的电子笔记本

• 批准号: 8361051
• 负责人: STEVEN J LUDTKE
• 金额: $ 6.13万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361051
• 关键词: 3-Dimensional; Archives; Development; Electron Microscopy; Electronics; Funding; Grant; Ice; Maps; Metadata; National Center for Research Resources; Play; Principal Investigator; Process; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Role; Source; Staging; Structure; United States National Institutes of Health; Work; cost; data format; density; macromolecule; particle; research study; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cryo-EM is a lengthy experimental and computational process, leading to a final 3-D map and related annotations. Each of the steps requires different expertise and skills. All of the investigators perform experiments somewhat differently in order to make each project work, and some manage to complete projects faster than others. In our Center, there are dozens of projects ongoing in any given month, each with different technical challenges and at different stages of development or progress. Some projects take only a few days to achieve a subnanometer resolution structure while others may take months or years to reach the same point. It is obvious that, in addition to various experimental and computational variables, investigators play a critical role in getting the project completed in a timely fashion. There are two aims in this core project. One aim is to archive all the metadata related to each particle from its initial embedding in ice to the final density map. The second aim is to develop an archival data format capable of amalgamating density maps and associated metadata.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 Cryo-EM是一个冗长的实验和计算过程，导致最终的3D图和相关注释。每个步骤都需要不同的专业知识和技能。所有调查人员的执行实验都有所不同，以使每个项目都起作用，并且有些人设法比其他项目更快地完成项目。在我们的中心，在任何给定月份都有数十个正在进行的项目，每个项目都面临着不同的技术挑战，并且在开发或进步的不同阶段。一些项目只需要几天才能达到亚纳米分辨率结构，而另一些项目可能需要几个月或几年才能达到同一点。显然，除了各种实验和计算变量外，研究人员在及时完成项目方面发挥了关键作用。这个核心项目有两个目标。一个目的是将与每个粒子的初始嵌入到冰中到最终密度图相关的所有元数据。第二个目的是开发能够合并密度图和相关元数据的档案数据格式。