WILLIAM WEIS PRT TIME: HIV

威廉·韦斯 PRT 时间：艾滋病毒

• 批准号: 7954167
• 负责人: William I Weis
• 金额: $ 0.26万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954167
• 关键词: Adherens Junction; Adhesions; Antigen-Presenting Cells; Architecture; Area; CD209 gene; Cadherins; Carbohydrates; Cell Adhesion Molecules; Cell membrane; Cell surface; Cells; Complement; Computer Retrieval of Information on Scientific Projects Database; Cytolysis; Cytoskeleton; Docking; Funding; Grant; HIV; Immune system; Institution; Intercellular Junctions; Investigation; Mannose Binding Lectin; Mediating; Membrane; Pathway interactions; Proteins; Research; Research Personnel; Resources; Serum; Signal Pathway; Source; Specificity; T-Cell Activation; T-Lymphocyte; Time; United States National Institutes of Health; Vesicle; beta catenin; computerized data processing; link protein; programs; protein structure; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This program aims to determine the structures of proteins involved in cell membrane targeting, adhesion and signaling processes, in order to establish the mechanism and specificity of their interactions. There are three principal areas under investigation, all of which with crystals that require synchrotron radiation. 1. Cell-surface carbohydrate recognition in the immune system, focusing on serum Mannose-binding Proteins and how they trigger complement-mediated cell lysis, and DC-SIGN, an adhesion molecule that mediates activation of T cells by antigen-presenting cells, and which also serves to capture HIV and deliver it to T cells. 2. Architecture and assembly of intercellular junctions, studying the interactions of the proteins that link cadherin cell adhesion molecules to the cytoskeleton. The adherens junction protein beta-catenin is also an essential component of the Wnt signaling pathway, and its interactions with other Wnt pathway components are also being studied. 3. Proteins responsible for specific docking and fusion of intracellular vesicles with target membranes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目旨在确定参与细胞膜靶向、粘附和信号传导过程的蛋白质的结构，以建立它们相互作用的机制和特异性。 目前正在研究三个主要领域，所有这些领域都含有需要同步辐射的晶体。 1. 免疫系统中的细胞表面碳水化合物识别，重点关注血清甘露糖结合蛋白及其如何触发补体介导的细胞裂解，以及 DC-SIGN（一种介导抗原呈递细胞激活 T 细胞的粘附分子），以及它还可以捕获 HIV 并将其传递给 T 细胞。 2. 细胞间连接的结构和组装，研究将钙粘蛋白细胞粘附分子与细胞骨架连接起来的蛋白质的相互作用。 粘附连接蛋白β-连环蛋白也是Wnt信号通路的重要组成部分，其与其他Wnt通路组分的相互作用也在研究中。 3.负责细胞内囊泡与靶膜的特异性对接和融合的蛋白质。