PILATUS3 X 1M X-ray detector

PILATUS3 X 1M X射线探测器

• 批准号: 9074860
• 负责人: William I Weis
• 金额: $ 55.01万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9074860
• 关键词: Biological; Biomedical Research; Catalysis; Communities; Complex; Coupled; Cryoelectron Microscopy; Crystallography; Data; Funding; Growth; Health; Human; International; Measurement; Methods; Molecular Sieve Chromatography; Mutation; Noise; Optics; Photons; Proteins; Reading; Research; Research Personnel; Resolution; Roentgen Rays; Signal Transduction; Silicon; Source; Structure; System; Technology; Time; United States National Institutes of Health; data acquisition; detector; drug development; improved; macromolecular assembly; macromolecule; protein protein interaction; research study; structural biology; synchrotron radiation; time use; tool

 DESCRIPTION (provided by applicant): Biological small angle x-ray scattering and diffraction (SAXS) provides structural and dynamic information from solutions or partially ordered arrays of biomolecules in physiologically relevant conditions, albeit at lower resolution (~7-10 Å or higher compared to that obtainable from crystallography or cryo-electron microscopy. SAXS is not only a powerful tool to study structures of large macromolecular assemblies in solution, but can also be used for time-resolved studies to investigate large conformational changes that occur during enzymatic catalysis, protein- protein interactions, and folding of macromolecules. Such studies are critical for biomedical research, as they provide basic mechanistic information that can be used to understand the effects of deleterious mutations, and can thereby inform drug development that ultimately impacts human health. The application of SAXS methods to problems in structural biology has seen tremendous growth in recent years, reflecting the need within the research community for experimental methods that can give structural and dynamic information on large multi-domain complexes. The explosive growth of SAXS has largely been enabled by technical advances that include high- brightness, low divergence X-ray sources, advances in X-ray optics, and X-ray detectors. As protein solutions scatter X-rays quite weakly, improved X-ray detectors are a critical enabling technology. The silicon pixel array X-ray detectors are ideally suited for SAXS, due to their zero read noise and photon counting technology, which open up new possibilities for SAXS applications in structural biology. This application is a request for a complete detector system for small angle X-ray scattering, consisting of a PILATUS3 X 1M Pixel Array Detector (PAD) manufactured by Dectris, Inc., and associated control, data acquisition, data handling and storage computing hardware. This system will be installed on beam line 4-2 (BL4-2) at the Stanford Synchrotron Radiation Lightsource (SSRL). The PILATUS3 1M PAD offers many advantages over the presently available detectors installed at the beam line including superior signal-to-noise, increased maximum frame rate, and larger dynamic range. The detector is well suited for size-exclusion chromatography coupled SAXS experiments, time-resolved, and high-throughput static SAXS measurements that will be needed to drive the forefront of BL4-2 research in structural biology on challenging biomedically important systems. It will maintain international competitiveness for NIH funded researchers.

 描述（由申请人提供）：生物小角 X 射线散射和衍射 (SAXS) 在生理相关条件下提供来自溶液或生物分子部分有序阵列的结构和动态信息，尽管分辨率较低（~7-10 Å 或更高）与从晶体学或冷冻电子显微镜中获得的结果相比，SAXS 不仅是研究溶液中大分子组装体结构的强大工具，而且还可用于时间分辨研究研究酶催化、蛋白质-蛋白质相互作用和大分子折叠过程中发生的大构象变化，此类研究对于生物医学研究至关重要，因为它们提供了可用于了解有害突变影响的基本机制信息。近年来，SAXS 方法在结构生物学问题上的应用有了巨大的增长，反映出研究界对能够提供大量结构和动态信息的实验方法的需求。 SAXS 的爆炸性增长很大程度上得益于技术进步，包括高亮度、低发散 X 射线源、X 射线光学的进步以及 X 射线探测器。相对较弱的是，改进的 X 射线探测器是一项关键的支持技术，由于其零读取噪声和光子计数技术，硅像素阵列 X 射线探测器非常适合 SAXS，这为 SAXS 应用开辟了新的可能性。该应用要求用于小角度 X 射线散射的完整探测器系统，包括 Dectris, Inc. 制造的 PILATUS3 X 1M 像素阵列探测器 (PAD) 以及相关的控制、数据采集、数据处理和该系统将安装在斯坦福同步辐射光源 (SSRL) 的光束线 4-2 (BL4-2) 上，与 PILATUS3 1M PAD 相比具有许多优势。目前安装在光束线上的检测器具有出色的信噪比、更高的最大帧速率和更大的动态范围，该检测器非常适合尺寸排阻色谱耦合 SAXS 实验、时间分辨和高通量静态实验。 SAXS 测量需要推动 BL4-2 结构生物学研究的前沿，以应对具有挑战性的生物医学重要系统，这将为 NIH 资助的研究人员保持国际竞争力。