STRUCTURAL STUDIES OF MTR4

MTR4 的结构研究

• 批准号: 8362287
• 负责人: SEAN J JOHNSON
• 金额: $ 0.16万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362287
• 关键词: Complex; DNA-Directed RNA Polymerase; Funding; Grant; Home environment; National Center for Research Resources; Pathway interactions; Play; Poly(A) Tail; Principal Investigator; Proteins; RNA; RNA Degradation; RNA Helicase; Radiation; Research; Research Infrastructure; Resources; Role; Source; Structure; United States National Institutes of Health; Zinc Fingers; cost; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The primary subject of this project is the Mtr4 protein, a highly conserved and essential eukaryotic protein that plays a central role in RNA surveillance and decay pathways. Mtr4 is an RNA helicase that acts in complex with Trf4 (an RNA polymerase) and Air2 (a zinc finger protein) to form the ?TRAMP? complex. By an unknown mechanism, this complex identifies RNAs for degradation, tags them with a short poly-A tail, and then delivers them to the exosome for exonucleolytic degradation. In order to elucidate the structural details of TRAMP-dependent RNA surveillance, we are determining crystal structures of Mtr4 and relevant complexes. Our current Mtr4 crystals diffract poorly on a home source, requiring synchrotron radiation for structure determination.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目的主要主题是MTR4蛋白，这是一种高度保守和必需的真核蛋白，在RNA监测和衰减途径中起着核心作用。 MTR4是与TRF4（RNA聚合酶）和AIR2（锌指蛋白）形成的RNA解旋酶以形成？流浪汉吗？复杂的。通过一种未知的机制，该复合物识别出降解的RNA，用短多A尾标记它们，然后将它们输送到外泌体以进行外核解水解降解。为了阐明流浪汉依赖性RNA监视的结构细节，我们正在确定MTR4和相关复合物的晶体结构。我们当前的MTR4晶体在家庭源上衍射较差，需要同步辐射来确定结构。