STRUCTURAL STUDIES OF MTR4

MTR4 的结构研究

• 批准号: 8170288
• 负责人: SEAN J JOHNSON
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170288
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; DNA-Directed RNA Polymerase; Funding; Grant; Home environment; Institution; Pathway interactions; Play; Poly(A) Tail; Proteins; RNA; RNA Degradation; RNA Helicase; Research; Research Personnel; Resources; Role; Source; Structure; United States National Institutes of Health; Zinc Fingers; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary subject of this project is the Mtr4 protein, a highly conserved and essential eukaryotic protein that plays a central role in RNA surveillance and decay pathways. Mtr4 is an RNA helicase that acts in complex with Trf4 (an RNA polymerase) and Air2 (a zinc finger protein) to form the ?TRAMP? complex. By an unknown mechanism, this complex identifies RNAs for degradation, tags them with a short poly-A tail, and then delivers them to the exosome for exonucleolytic degradation. In order to elucidate the structural details of TRAMP-dependent RNA surveillance, we are determining crystal structures of Mtr4 and relevant complexes. Our current Mtr4 crystals diffract poorly on a home source, requiring synchrotron radiation for structure determination.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的主要主题是 Mtr4 蛋白，这是一种高度保守且必需的真核蛋白，在 RNA 监视和衰变途径中发挥核心作用。 Mtr4 是一种 RNA 解旋酶，与 Trf4（一种 RNA 聚合酶）和 Air2（一种锌指蛋白）复合作用，形成“TRAMP”复杂的。通过一种未知的机制，该复合物识别要降解的 RNA，用短聚 A 尾标记它们，然后将它们递送到外泌体进行核酸外切降解。为了阐明 TRAMP 依赖性 RNA 监视的结构细节，我们正在确定 Mtr4 和相关复合物的晶体结构。我们目前的 Mtr4 晶体在家用光源上的衍射性能很差，需要同步加速器辐射来确定结构。