PRMT1 MEDIATED ARG METHYLATION OF INHIBITORY SMADS IN TGF-BETA SIGNALLING

PRMT1 介导 TGF-β 信号传导中抑制性 SMAD 的 ARG 甲基化

• 批准号: 8363822
• 负责人: RIK M DERYNCK
• 金额: $ 1.03万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363822
• 关键词: Arginine; Development; Funding; Goals; Grant; Mass Spectrum Analysis; Mediating; Methylation; Molecular; National Center for Research Resources; Principal Investigator; Regulation; Research; Research Infrastructure; Resources; Role; Signal Transduction; Source; Transforming Growth Factor beta; United States National Institutes of Health; base; cost; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project aims to define the role of PRMT1 mediated arginine methylation of inhibitory Smads in the regulation of TGF-beta/BMP signaling. We aim to elucidate the molecular mechanism underlying PRMT1 mediated arginine methylation through identifying specific arginine resudes that are methylated, and examine the alteration of signaling response when this methylation is ablated. Assistance from UCSF Mass Spectrometry Facility is critical for achieving our research goals in that mass spectrometric analysis will help us identify target arginine resudes and confirm their methylation status. This will serve as the basis for our project development.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目旨在定义PRMT1介导的抑制性SMAD的精氨酸甲基化在TGF-β/BMP信号调节中的作用。 我们的目的是通过鉴定特定的精氨酸重塑被甲基化的特定精氨酸，并检查这种甲基化消融时，通过鉴定特定的精氨酸来阐明了PRMT1介导的精氨酸甲基化的分子机制。 UCSF质谱设施的援助对于实现我们的研究目标至关重要，因为质谱分析将有助于我们确定目标精氨酸的分离并确认其甲基化状态。 这将是我们项目开发的基础。