PRMT1 MEDIATED ARG METHYLATION OF INHIBITORY SMADS IN TGF-BETA SIGNALLING

PRMT1 介导 TGF-β 信号传导中抑制性 SMAD 的 ARG 甲基化

• 批准号: 8169818
• 负责人: RIK M DERYNCK
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169818
• 关键词: Arginine; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Goals; Grant; Institution; Mass Spectrum Analysis; Mediating; Methylation; Molecular; Regulation; Research; Research Personnel; Resources; Role; Signal Transduction; Source; Transforming Growth Factor beta; United States National Institutes of Health; base; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to define the role of PRMT1 mediated arginine methylation of inhibitory Smads in the regulation of TGF-beta/BMP signaling. We aim to elucidate the molecular mechanism underlying PRMT1 mediated arginine methylation through identifying specific arginine resudes that are methylated, and examine the alteration of signaling response when this methylation is ablated. Assistance from UCSF Mass Spectrometry Facility is critical for achieving our research goals in that mass spectrometric analysis will help us identify target arginine resudes and confirm their methylation status. This will serve as the basis for our project development.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目旨在定义PRMT1介导的抑制性SMAD的精氨酸甲基化在TGF-β/BMP信号调节中的作用。 我们的目的是通过鉴定特定的精氨酸重塑被甲基化的特定精氨酸，并检查这种甲基化消融时，通过鉴定特定的精氨酸来阐明了PRMT1介导的精氨酸甲基化的分子机制。 UCSF质谱设施的援助对于实现我们的研究目标至关重要，因为质谱分析将有助于我们确定目标精氨酸的分离并确认其甲基化状态。 这将是我们项目开发的基础。