DESIGN OF SELF-ASSEMBLED 3D DNA CRYSTALS USING 6HB

使用 6HB 设计自组装 3D DNA 晶体

• 批准号: 7957272
• 负责人: JENS J BIRKTOFT
• 金额: $ 1.88万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957272
• 关键词: Biological; Computer Retrieval of Information on Scientific Projects Database; Crystallography; DNA; Devices; Diffusion; Electronics; Funding; Goals; Grant; Institution; Light; Methods; Molecular; Nanotechnology; Pattern; Positioning Attribute; Proteins; Research; Research Personnel; Resolution; Resources; Shapes; Source; Synchrotrons; System; United States National Institutes of Health; Work; X ray diffraction analysis; X-Ray Diffraction; base; design; macromolecule; nanoscale; success; three dimensional structure; vapor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Structural DNA nanotechnology uses unusual DNA motifs to build target shapes and periodic arrangements. We expect these systems can be applied to several practical ends: The key motivating goal for this research is that spatially periodic networks are crystals. Also the Steepest challenge of the research is the construction of 3D crystals with high order. If we can build stick-figure crystalline cages in the nanometer scale, they could be used to orient other biological macromolecules as guests inside those cages, thereby rendering their 3D structure amenable to diffraction analysis. The globular shapes of proteins are not conducive to the packing alignment that is essential to form a well-ordered crystal, due to specific interactions between DNA and proteins, DNA 3D crystalline crystal is an excellent candidate for forming periodic crystalline cages that can host proteins in order for the X-ray diffraction studies; Similarly, the same crystalline arrays could be used to position and orient components of molecular electronic devices with nanometer-scale precision. A number of designs using unusual DNA motifs have been self-assembled to yield 3D crystals. Although we have been successfully build 2D arrays, the structural criteria of success with 2D arrangements are based typically on AFM observation with resolution limit of 3-10 nm. By contrast the goal for successful work of x-ray diffraction is around 2 ¿¿¿ . However the diffraction patterns of our preliminary crystals have been limited to 10 ¿¿¿ . Six-DNA helix-Bundle is the exact motif I¿¿¿¿¿"m using for this research. In this motif six DNA helices associate to form a hexagon arrangement if you look down the helical axis of DNA. We use thermo-control and vapour-diffusion methods. We have been successfully made 2D arrays by using the former method.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以出现在其他 CRISP 条目中 列出的机构是。 对于中心来说，它不一定是研究者的机构。 结构 DNA 纳米技术使用不寻常的 DNA 基序来构建目标形状和周期性排列，我们期望这些系统可以应用于几个实际目的：这项研究的关键动机目标是空间周期性网络是晶体。构建高阶 3D 晶体 如果我们能够构建纳米尺度的简笔画晶体笼，它们可以用来定向其他生物大分子作为这些笼内的客体，从而呈现它们的 3D 效果。蛋白质的球状结构不利于形成有序晶体所必需的堆积排列，由于 DNA 和蛋白质之间的特定相互作用，DNA 3D 晶体是形成周期性晶体的绝佳候选者。可以容纳蛋白质以进行 X 射线衍射研究；同样，相同的晶体阵列可用于以纳米级精度定位和定向分子电子设备的组件。 许多使用不寻常 DNA 基序的设计已被自组装以产生 3D 晶体，尽管我们已经成功构建了 2D 阵列，但 2D 排列成功的结构标准通常基于分辨率限制为 3-10 nm 的 AFM 观察。相比之下，X 射线衍射成功工作的目标约为 2 ¿ ¿ ¿然而，我们初步晶体的衍射图被限制在 10 ¿ ¿ ¿ . 六 DNA 螺旋束是确切的基序 I¿ ¿ ¿ ¿ ¿ “我用于这项研究。在这个图案中，如果你向下看 DNA 的螺旋轴，六个 DNA 螺旋会联合形成六边形排列。我们使用温度控制和蒸汽扩散方法。我们已经通过使用以前的方法。