SYSTEMATIC GROWTH OF PROTEIN CRYSTALS FOR HIGH-RESOLUTION CRYSTALLOGRAPHIC AN

用于高分辨率晶体学的蛋白质晶体的系统生长

• 批准号: 8171990
• 负责人: Anna S Gardberg
• 金额: $ 0.73万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171990
• 关键词: Address; Binding Proteins; Biological Assay; Computer Retrieval of Information on Scientific Projects Database; Crystallization; Crystallography; Enzymes; Funding; Grant; Growth; Institution; Medical; Membrane; Neutrons; Pharmacologic Substance; Proteins; Research; Research Personnel; Resolution; Resources; Robotics; Screening procedure; Signal Transduction; Source; System; Techniques; Time; United States National Institutes of Health; Work; interest; structural genomics; ultra high resolution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our work aims to address a bottleneck in crystallography - the systematic growth of single crystals of soluble and membrane bound proteins that are suitable for ultra-high resolution x-ray and neutron studies. We aim to address this problem by using the high throughput, robotic protein crystallization screening techniques that have facilitated screening and crystal growth in the structural genomics world. The effects of varying multiple parameters can be screened and evaluated rapidly, systematically and with dozens of proteins assayed against 1000?s of variables at the same time. We are studying more than 30 different protein systems, including cellular, signaling and membrane bound proteins and enzymes of interest in medical, pharmaceutical, industrial, bio-defense and bio-energy research.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们的工作旨在解决晶体学的瓶颈——适用于超高分辨率 X 射线和中子研究的可溶性和膜结合蛋白单晶的系统生长。我们的目标是通过使用高通量机器人蛋白质结晶筛选技术来解决这个问题，这些技术促进了结构基因组学领域的筛选和晶体生长。可以快速、系统地筛选和评估改变多个参数的影响，并同时针对 1000 个变量分析数十种蛋白质。我们正在研究 30 多种不同的蛋白质系统，包括医学、制药、工业、生物防御和生物能源研究中感兴趣的细胞、信号和膜结合蛋白和酶。