MICRO-CT OF CARDIAC FUNCTION IN RAT WITH DOBUTAMINE CHALLENGE

多巴酚丁胺挑战大鼠心脏功能的显微 CT

• 批准号: 8363163
• 负责人: BRAIN BERRIDGE
• 金额: $ 1.84万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363163
• 关键词: Animals; Biological; Biological Testing; Blood; Calibration; Cardiac; Cardiac Output; Cardiac Volume; Chronology; Clinical; Data; Dobutamine; EFRAC; Error Sources; Funding; Generations; Gold; Grant; Image; Intravenous infusion procedures; Left; Life; Measures; Methods; Microscopy; Myocardium; National Center for Research Resources; Noise; Physiology; Principal Investigator; Protocols documentation; Rattus; Relative (related person); Reporting; Research; Research Design; Research Infrastructure; Resources; Scanning; Source; Stroke Volume; System; Techniques; Testing; Thick; Time; United States National Institutes of Health; X-Ray Computed Tomography; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Study Design -- Animals: approx. 6 week old CD rats (250 gm); n = 6 -- Test article: Dobutamine, 30 ug/kg/min IV infusion Quantitative endpoints- total cardiac volume; left, right, and septal wall thickness; EDV, ESV, ejection fraction, stroke volume, cardiac output, fractional shortening Imaging: All imaging will be performed on the 1st-generation micro-CT system at the Duke CIVM. We will acquire 12 points in the cardiac cycle for 6 animals at two different times separated by at least one week. Each session will include a baseline followed by a challenge with dobutamine. Chronology: 2 animals imaged/day X 3 days. Animals will be returned to their cages for one week to recover to a baseline physiology and the study will be repeated. Phantom Calibrations: We will validate the CT scanning protocol and analysis using a phantom with objects of accurately known volumes. A (static) phantom will be constructed with CT (Hounsfield) differences comparable to those of the animal (myocardium/blood pool). The volumes of the phantom objects will be know with accuracy of at least 1%. The phantom will be scanned with techniques that produce contrast to noise at least 2X better than that seen in the clinical (live animal) setting. We will digitally alter the image sets to produce images with contrast to noise @ 0.5X, 1.0 X and (original) 2.0 X the CNR. This will allow us to separate any sources of errors from scanning and segmentation from the biological variability we wish to measure. Analysis: Phantom data will analyzed with three independent approaches : --- Autosegmentation using Vitrea cardiac functional analysis --- Autosegmentation using the PSC browser --- Semiautomated segmentation using Image J In all cases, the error will be reported relative to the gold standard (static phantom). At this point we may choose one or both methods to apply to the biological test data.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 研究设计 - 动物：大约6周大的CD大鼠（250克）； n = 6 - 测试文章：多丁胺，30 ug/kg/min IV输注 定量终点 - 总心脏量；左，右，隔膜厚度； EDV，ESV，射血分数，中风量，心输出量，分数缩短 成像：所有成像将在杜克群岛的第一代微型CT系统上执行。 我们将在两次不同的时间中至少一周的两次不同时间，在心脏周期中为6只动物获得12分。每个课程将包括基线，然后对多丁胺进行挑战。 年表：2只动物/X天3天。动物将被送回笼子一周，以恢复基线生理，并将重复研究。 幻影校准：我们将使用具有准确已知体积的对象的幻象来验证CT扫描方案和分析。 （静态）幻影将使用与动物（心肌/血池）相当的CT（Hounsfield）差异构建。幻影对象的体积至少为1％。幻影将用比临床（活动物）环境中的噪声更好的噪声来扫描，与噪声形成鲜明对比至少2倍。我们将在数字上更改图像集以产生与噪声 @ 0.5x，1.0 x和（原始）2.0 x CNR形成鲜明对比的图像。这将使我们能够将任何错误来源与扫描和分割分开，并将其与希望测量的生物变异性分开。 分析：幻影数据将通过三种独立的方法进行分析： ---使用Vitrea心脏功能分析进行自动分量 ---使用PSC浏览器进行自动分组 ---使用图像j的半小节分割 在所有情况下，误差将相对于黄金标准（静态幻影）报告。在这一点上，我们可以选择一种或两种方法应用于生物测试数据。