MOUSE BRAIN ATLAS

小鼠大脑图谱

• 批准号: 8363148
• 负责人: George ALLAN JOHNSON
• 金额: $ 1.84万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363148
• 关键词: Brain; Data; Data Set; Funding; Goals; Grant; Histologic; Image; Maps; Microscopy; Mus; National Center for Research Resources; Optics; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Source; Staining method; Stains; United States National Institutes of Health; Weight; Work; cost; cresyl violet

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our previous work with actively stained mouse brains has allowed us to see the cytoarchitecture down to 20 microns. But, our understanding of the sources of contrast at this level of resolution is quite primative. The goal of this project is to understand more fully the sources of contrast of the cytoarchitecture in the actively stained mouse brain using the 9T MR magnet. 1. We will acquire quantitative T1 and T2 maps of the mouse brain at 40 microns with accompanying higher resolution spin echo (T1-weighted) images for definition of the cytoarchitecture. 2. We will histologically section the same brains and register the resulting datasets to produce 3D optical sectioned data with Nissl and Cresyl violet. 3. MR and optical sections will be registered into single volumes. 4. The resulting data will be used to define the specific sources of contrast in the most critical regions of the actively stained brain.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 我们之前对小鼠大脑进行主动染色的研究使我们能够看到小至 20 微米的细胞结构。但是，我们对这种分辨率水平的对比度来源的理解还相当原始。该项目的目标是使用 9T MR 磁铁更全面地了解主动染色小鼠大脑中细胞结构的对比来源。 1. 我们将获取 40 微米处小鼠大脑的定量 T1 和 T2 图，并附有更高分辨率的自旋回波（T1 加权）图像，以定义细胞结构。 2. 我们将对相同的大脑进行组织学切片并记录所得数据集，以使用尼氏和甲酚紫生成 3D 光学切片数据。 3. MR 和光学切片将注册成单卷。 4. 所得数据将用于定义活跃染色大脑最关键区域的特定对比度来源。