ISOLATION AND STRUCTURAL CHARACTERIZATION OF MARINE FUNGAL SIDEROPHORES

海洋真菌铁载体的分离和结构表征

• 批准号: 8359634
• 负责人: Jessica Elanor Martin
• 金额: $ 13.58万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359634
• 关键词: Amino Acids; Antarctic; Bacteria; Binding; Biomedical Research; Cells; Data; Environment; Fatty Acids; Funding; Future; Grant; Growth; Iron; Marines; Mass Spectrum Analysis; Methods; Microbe; Molecular Weight; National Center for Research Resources; Oklahoma; Organism; Penicillium; Principal Investigator; Production; Research; Research Infrastructure; Resources; Siderophores; Source; Structure; Techniques; United States National Institutes of Health; Work; base; cost; fungus; hydroxamate; microbial; microorganism; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Background: Many microorganisms produce low-molecular-weight, iron(III) specific binding compounds called siderophores to compete for iron. Microbes are thought to produce unique siderophore structures to successfully compete for iron against other organisms in their environment. The siderophores isolated from terrestrial bacteria and fungi and from marine bacteria show promise as biomedical agents and for control of microbial growth. The siderophores produced by marine fungi have not been characterized and thus provide an unexplored source of novel bioactive compounds. Hypothesis: Marine-derived fungi produce siderophores with novel structures. Methods: Marine-derived fungal strains are screened for production of siderophores in pure cultures under conditions of iron limitation. Siderophores are isolated and structurally characterized using amino acid analysis, fatty acid analysis, NMR, mass spectrometry, and other techniques, as appropriate. Results and future directions: Twelve marine fungal strains have been screened for siderophore production; eleven of the twelve were found to produce siderophores. Structural analysis of siderophores produced by three strains is currently underway. One secreted siderophore has been isolated from an Antarctic strain of Leucosporidium. One secreted hydroxamate siderophore and two cell-associated siderophores have been isolated from Sporidiobolus salmonicolor 05-001. One secreted siderophore was isolated from Penicillium raistrickii ATCC42470. Mass spectrometric and NMR analyses of these siderophores has been completed. Current work focuses on structure determination based on NMR and mass spectrometric data.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 背景：许多微生物产生低分子量的铁（III）特异性结合化合物（称为铁载体）来竞争铁。人们认为微生物能够产生独特的铁载体结构，以成功地与环境中的其他生物体竞争铁。从陆地细菌和真菌以及海洋细菌中分离的铁载体显示出作为生物医学制剂和控制微生物生长的前景。海洋真菌产生的铁载体尚未被表征，因此提供了新型生物活性化合物的未经探索的来源。 假设：海洋来源的真菌产生具有新颖结构的铁载体。 方法：筛选海洋来源的真菌菌株，以在铁限制条件下在纯培养物中产生铁载体。使用氨基酸分析、脂肪酸分析、NMR、质谱和其他适当的技术来分离铁载体并进行结构表征。 结果和未来方向：已筛选出 12 种海洋真菌菌株来生产铁载体；十二个中的十一个被发现产生铁载体。目前正在对三种菌株产生的铁载体进行结构分析。从白孢子虫南极菌株中分离出一种分泌性铁载体。已从鲑鱼孢子虫 05-001 中分离出一种分泌的异羟肟酸铁载体和两种细胞相关铁载体。从Penicillium raistrickii ATCC42470 中分离出一种分泌性铁载体。这些铁载体的质谱和核磁共振分析已经完成。目前的工作重点是基于核磁共振和质谱数据的结构测定。