NOVEL SIDEROPHORES PRODUCED BY MARINE BACTERIA AND FUNGI

海洋细菌和真菌产生的新型铁载体

• 批准号: 7960031
• 负责人: Jessica Elanor Martin
• 金额: $ 9.23万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960031
• 关键词: Amino Acids; Bacteria; Binding; Biological Assay; Biomedical Research; Computer Retrieval of Information on Scientific Projects Database; Cunninghamella; Environment; Fatty Acids; Funding; Future; Grant; Growth; Institution; Iron; Marines; Mass Spectrum Analysis; Methods; Microbe; Molecular Weight; Oklahoma; Organism; Production; Research; Research Personnel; Resources; Screening procedure; Siderophores; Source; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry; Structure; Techniques; Time; United States National Institutes of Health; Work; Yeasts; base; fungus; microbial; microorganism; novel; research study; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Many microorganisms produce low-molecular-weight, iron(III)-specific binding compounds called siderophores to compete for iron. Microbes are thought to produce unique siderophore structures to successfully compete for iron against other organisms in their environment. The siderophores isolated from terrestrial bacteria and fungi and from marine bacteria show promise as biomedical agents and for control of microbial growth. The siderophores produced by marine fungi have not been characterized and thus provide an unexplored source of novel bioactive compounds. Hypothesis: Marine-derived fungi produce siderophores with novel structures. Methods: Marine-derived fungal strains are screened for production of siderophores in pure cultures under conditions of iron limitation. Siderophores are isolated and structurally characterized using amino acid analysis, fatty acid analysis, NMR, mass spectrometry, and other techniques, as appropriate. Results and future directions: At this time, we have identified Cunninghamella elegans ATCC 36112 as a siderophore producing marine-derived fungus. A novel siderophore has been isolated from the fungus and characterized using UV-Visible spectrophotometry, electrospray ionization mass spectrometry, and tandem mass spectrometry. We are currently working to purify sufficient material for analysis by NMR, which will allow us to complete the structural determination for this novel siderophore. We have also begun screening several strains of marine-derived yeasts. These strains appear to produce siderophores (based on Fe(III)-CAS assay) and we will begin purification of the compounds immediately. Experiments planned in the immediate future will isolate and structurally characterize siderophores produced by these new strains.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 背景：许多微生物产生低分子量，铁（III）特异性结合化合物，称为铁载体以争夺铁。 人们认为微生物会产生独特的辅助载体结构，以成功竞争其环境中其他生物体的铁。 从陆生细菌和真菌中分离出来的铁载体以及海洋细菌显示出有望作为生物医学剂并控制微生物生长。 海洋真菌产生的铁载体尚未被表征，因此提供了新型生物活性化合物的源头。 假设：海洋来源的真菌产生具有新结构的铁载体。 方法：在铁限制的条件下，筛选了海洋来源的真菌菌株以生产纯培养物中的铁载体。 使用氨基酸分析，脂肪酸分析，NMR，质谱法和其他技术在结构上分离并在结构上表征铁载体。 结果和未来的方向：目前，我们已经确定坎宁安娜秀丽隐杆线虫ATCC 36112是生产海洋衍生真菌的铁素载体。 已经从真菌中分离出一种新型的铁载体，并使用紫外可见分光光度计，电喷雾电离质谱法和串联质谱法进行了表征。 我们目前正在努力通过NMR净化足够的材料来分析，这将使我们能够完成对这种新型铁体的结构确定。 我们还开始筛选几种海洋来源的酵母菌。 这些菌株似乎会产生铁载体（基于Fe（III）-CAS分析），我们将立即开始纯化化合物。 在不久的将来计划的实验将隔离和结构表征这些新菌株产生的铁载体。