IRON-BINDING COMPOUNDS PRODUCED BY OPEN-WATER MARINE FUNGI

开放水域海洋真菌产生的铁结合化合物

• 批准号: 7960032
• 负责人: Jessica Elanor Martin
• 金额: $ 0.95万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960032
• 关键词: Amino Acids; Bacteria; Binding; Biological Factors; Biomedical Research; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Fatty Acids; Funding; Grant; Growth; High Pressure Liquid Chromatography; Institution; Iron; Learning; Marines; Mass Spectrum Analysis; Methods; Microbiological Techniques; Molecular Weight; Oceans; Oklahoma; Phase; Production; Research; Research Personnel; Resources; Siderophores; Solid; Source; Structure; Students; Techniques; Training and Education; United States National Institutes of Health; Universities; Water; fungus; microbial; microorganism; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many microorganisms produce low-molecular-weight, iron(III)-specific binding compounds called siderophores to compete for iron during iron-limitation. The siderophores isolated from terrestrial bacteria and fungi and from marine bacteria show promise as biomedical agents and for control of microbial growth. To date, no iron-binding compounds produced by marine fungi have been structurally characterized. The siderophores produced by marine fungi provide an as yet unexplored source of novel bioactive compounds. The proposed project will provide the first examples of marine fungal siderophore structures. The specific aims of this project are to: 1. Screen open-ocean fungal strains for the production of iron-binding compounds under conditions of iron limitation. 2. Isolate iron-binding compounds from open-ocean fungal strains. 3. Determine the structure(s) of siderophore(s) produced by open-ocean fungi using amino acid analysis, NMR, and mass spectrometry. Involvement of undergraduate students in research is a vital component of their training and education. The proposed research will be accomplished in collaboration with undergraduate students from Northeastern State University. Students will have the opportunity to learn microbiological techniques, natural product isolation and purification techniques including solid phase extraction and reversed-phase HPLC, and structure characterization methods including amino acid analysis, fatty acid analysis, mass spectrometry, and 1D and 2D NMR.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 许多微生物产生低分子量的铁（III）特异性结合化合物，称为铁载体，以在铁限制期间竞争铁。 从陆生细菌和真菌中分离出来的铁载体以及海洋细菌显示出有望作为生物医学剂并控制微生物生长。 迄今为止，尚未在结构上表征海洋真菌产生的铁结合化合物。 海洋真菌生产的铁载体提供了一种尚未开发的新型生物活性化合物来源。 拟议的项目将提供海洋真菌铁载体结构的第一个例子。 该项目的具体目的是： 1。在铁限制条件下生产铁结合化合物的屏幕开放海洋真菌菌株。 2。来自开放海洋真菌菌株的分离型铁结合化合物。 3。确定使用氨基酸分析，NMR和质谱法产生的恒星载体的结构。 本科生参与研究是他们培训和教育的重要组成部分。 拟议的研究将与东北州立大学的本科生合作完成。 学生将有机会学习微生物技术，天然产物隔离和纯化技术，包括固相萃取和相反的相HPLC以及结构表征方法，包括氨基酸分析，脂肪酸分析，质谱和1D和2D NMR。