TOXIN INTERACTIONS WITH TRPV1

毒素与 TRPV1 的相互作用

• 批准号: 7957414
• 负责人: David Julius
• 金额: $ 0.6万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957414
• 关键词: Agonist; Collection; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Ion Channel; Mass Spectrum Analysis; Molecular; Molecular Weight; Organism; Proteins; Research; Research Personnel; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; TRPV1 gene; Toxin; United States National Institutes of Health; reversed phase chromatography; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One of the most powerful tools for analyzing ion channel structure/function has been the identification of natural toxins agonists and antagonists. Several organisms produce small protein toxins that act on ion channels and aid in prey capture or defense. We propose to study toxin interaction with the ion channel TRPV1. We will use MALDI mass spectrometry to assess the molecular composition of toxins isolated by reverse phase chromatography. The UCSF Mass Spectrometry Facility will assist in the collection to determine the molecular weight of isolated toxins.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 分析离子通道结构/功能的最强大工具之一是天然毒素激动剂和拮抗剂的鉴定。 几种生物体会产生小蛋白毒素，作用于离子通道并有助于捕获或防御猎物。 我们建议研究毒素与离子通道 TRPV1 的相互作用。 我们将使用 MALDI 质谱法来评估反相色谱法分离的毒素的分子组成。 加州大学旧金山分校质谱设施将协助收集以确定分离的毒素的分子量。