TOXIN INTERACTIONS WITH TRPV1

毒素与 TRPV1 的相互作用

• 批准号: 8363781
• 负责人: David Julius
• 金额: $ 0.03万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363781
• 关键词: Agonist; Collection; Funding; Grant; Ion Channel; Mass Spectrum Analysis; Molecular; Molecular Weight; National Center for Research Resources; Organism; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; TRPV1 gene; Toxin; United States National Institutes of Health; cost; reversed phase chromatography; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. One of the most powerful tools for analyzing ion channel structure/function has been the identification of natural toxins agonists and antagonists. Several organisms produce small protein toxins that act on ion channels and aid in prey capture or defense. We propose to study toxin interaction with the ion channel TRPV1. We will use MALDI mass spectrometry to assess the molecular composition of toxins isolated by reverse phase chromatography. The UCSF Mass Spectrometry Facility will assist in the collection to determine the molecular weight of isolated toxins.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 分析离子通道结构/功能的最强大工具之一是天然毒素激动剂和拮抗剂的鉴定。 几种生物体会产生小蛋白毒素，作用于离子通道并有助于捕获或防御猎物。 我们建议研究毒素与离子通道 TRPV1 的相互作用。 我们将使用 MALDI 质谱法来评估反相色谱法分离的毒素的分子组成。 加州大学旧金山分校质谱设施将协助收集以确定分离的毒素的分子量。