TRP CHANNEL MODULATION

TRP 通道调制

• 批准号: 8169782
• 负责人: David Julius
• 金额: $ 0.35万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169782
• 关键词: Binding; Biochemical; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Cytoplasmic Tail; Esthesia; Funding; Future; Grant; Institution; Mass Spectrum Analysis; Pain; Peptides; Proteins; Research; Research Personnel; Resources; Site; Source; United States National Institutes of Health; crosslink; small molecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to perform biochemical characterization of proteins that modulate the activity of channels important for pain sensation. The targets of study will include channel cytoplasmic domains, small molecule modulators, and peptide modulators. The objective is to purify proteins, determine molecular interaction sites, and perform structural studies.The UCSF mass spectrometry facility will aid us in determining the accurate mass of purified products and confirming the identity of purified products. The aid of the facility will also allow us to interpret results from chemical crosslinking studies in the future.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的目的是对调节痛觉重要通道活性的蛋白质进行生化表征。 研究目标包括通道胞质结构域、小分子调节剂和肽调节剂。 目的是纯化蛋白质、确定分子相互作用位点并进行结构研究。加州大学旧金山分校的质谱设施将帮助我们确定纯化产物的准确质量并确认纯化产物的身份。 该设施的帮助还将使我们能够在未来解释化学交联研究的结果。