IMAGING CORE

成像核心

基本信息

批准号：
8360504
负责人：
GRAZYNA RAJKOWSKA
金额：
$ 7.22万
依托单位：
UNIVERSITY OF MISSISSIPPI MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8360504
关键词：
3-Dimensional Animals Area Autopsy Behavior Brain Brain region California Cell Count Computer software Confocal Microscopy Equipment Fluorescence Fluorescent Antibody Technique Funding Grant Head Histology Human Human Resources Image Image Analysis Imagery Immunohistochemistry Instruction Laboratories Lasers Light Microscope Maintenance Measurement Medical center Mental disorders Methods Microscope Microscopic Microscopy Mississippi Molecular Morphology National Center for Research Resources Neuroanatomy Neuroglia Neurons Neurosciences Neurosciences Research Paper Principal Investigator Procedures Process Proteins Psychiatry Publishing Research Research Infrastructure Research Personnel Resources Sampling Scanning Slice Source Staining method Stains Supervision Synapses System Techniques Tissues Training United States National Institutes of Health Universities abstracting brain tissue cellular imaging cost cryostat density design digital experience insight investigator training laser capture microdissection neurochemistry neuropathology professor receptor binding

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. As is the case for all areas of neuroscience research, the application of modern methods of cellular imaging are greatly accelerating advancements in the field. The center's Imaging Core gives investigators access to state-of-the-art, sophisticated microscopic equipment with advanced capabilities in the visualization, quantification and interpretation of neuronal systems at the cellular level. Dr. Grazyna Rajkowska, (Professor of Psychiatry and Human Behavior; Head of the Quantitative Neuroanatomy Laboratory), serves as Core Director of the CPN's Imaging Core. Dr. Rajkowska is a neuroanatomist with 20 years experience in quantitative image analysis of specific types of neurons and glial cells in human postmortem and animal brain tissues. She has served as Head of the Laboratory of Quantitative Neuroanatomy at UMC for the past 13 years and has published over 100 scientific papers and abstracts in the field of quantitative morphology. Dr. Rajkowska provides oversight of investigator training in the use of core equipment, the maintenance of core equipment, and the supervision of core personnel. In addition to the administrative oversight of the Imaging Core, Dr. Rajkowska instructs Center investigators and other UMC researchers in the general use of the microscopic imaging equipment and further offers highly specialized expertise in the technical parameters relevant to the design, application and interpretation of quantitative morphological and neurochemical studies. These technical parameters include tissue slicing and general histological staining of human postmortem and animal brain tissues, the application of 3-dimensional cell counting techniques to different types of staining procedures, the determination of optimal conditions for immunohistochemistry and guidance in the anatomical localization of the specific brain regions being studied. Dr. Jose Javier Miguel-Hidalgo (Assistant Professor of Psychiatry & Human Behavior, University of Mississippi Medical Center) is a neuroanatomist with 18 years experience in microscopy, histology and histochemical processing of brain samples in a variety of vertebrate species. Dr. Miguel-Hidalgo received formal training in confocal microscopy in 1992 at the University of California at Davis. CPN and UMC investigators often use confocal microscopy for double immunostaining with fluorescent antibodies. Dr. Miguel-Hidalgo also received instruction directly from Arcturus technical field trainers in 2005 and has subsequently trained several researchers at UMC using laser capture microdissection in their research. He further assists Dr. Rajkowska in determining the technical parameters (tissue sectioning, measurement, sampling, etc.) that would produce the best images for each investigator's particular application. The CPN's Imaging Core and UMC currently support a Imaging Facility providing shared use of the following imaging equipment: + Nikon C1 Laser Scanning Confocal Microscope + Arcturus Laser Capture Microdissection System with fluorescence and 60X objective + Kodak Imaging Station + Fuji BAS-5000 Phosphor Image Analysis System + MCID Image Analysis Systems (2) + AIS Image Analysis Workstation + StereoInvestigator and Neurolucida Quantitative Cell Counting System + 3-D Cell Counting System (Williams & Rakic) + Bioquant Cell Counting and Imaging System + Nikon Light Microscope, with stereoinvestigator software + Leica Stereomicroscope + Zeiss Axioskop Microscopes (2) + Axioskop Digital Photomicroscopy Workstation. + Leica Cryostat These sophisticated imaging systems give investigators the ability to estimate, in three-dimensional space, numbers of cells, terminals, or synapses and to analyze the density of receptor binding and the level of specific proteins, giving researchers unprecedented insight into the cellular and molecular neuropathology of mental disorders.

该子项目是利用资源的众多研究子项目之一由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持并且子项目的主要研究者可能是由其他来源提供的，包括其他 NIH 来源。子项目可能列出的总成本代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。与神经科学研究的所有领域一样，现代细胞成像方法的应用正在极大地加速该领域的进步。该中心的成像核心使研究人员能够使用最先进、精密的显微设备，这些设备具有在细胞水平上对神经元系统进行可视化、量化和解释的先进功能。 Grazyna Rajkowska 博士（精神病学和人类行为学教授；定量神经解剖学实验室负责人）担任 CPN 影像核心的核心主任。 Rajkowska 博士是一位神经解剖学家，在人类死后和动物脑组织中特定类型的神经元和神经胶质细胞的定量图像分析方面拥有 20 年的经验。过去 13 年，她一直担任 UMC 定量神经解剖学实验室负责人，并在定量形态学领域发表了 100 多篇科学论文和摘要。 Rajkowska 博士负责监督研究者在核心设备的使用、核心设备的维护以及核心人员的监督方面的培训。除了对成像核心进行行政监督外，Rajkowska 博士还指导中心研究人员和其他 UMC 研究人员如何使用显微成像设备，并进一步提供与定量成像的设计、应用和解释相关的技术参数方面的高度专业知识。形态学和神经化学研究。这些技术参数包括人类死后和动物脑组织的组织切片和一般组织学染色、3维细胞计数技术在不同类型染色程序中的应用、免疫组织化学最佳条件的确定以及特定组织的解剖定位的指导。正在研究的大脑区域。 Jose Javier Miguel-Hidalgo 博士（密西西比大学医学中心精神病学和人类行为学助理教授）是一位神经解剖学家，在各种脊椎动物脑样本的显微镜、组织学和组织化学处理方面拥有 18 年经验。 Miguel-Hidalgo 博士于 1992 年在加州大学戴维斯分校接受了共焦显微镜的正式培训。 CPN 和 UMC 研究人员经常使用共聚焦显微镜进行荧光抗体双重免疫染色。 Miguel-Hidalgo 博士还于 2005 年直接接受了 Arcturus 技术领域培训师的指导，随后在 UMC 的几位研究人员的研究中使用激光捕获显微切割技术进行了培训。他进一步协助 Rajkowska 博士确定技术参数（组织切片、测量、采样等），以便为每位研究者的特定应用生成最佳图像。 CPN 的成像核心和 UMC 目前支持成像设施，提供以下成像设备的共享使用： + Nikon C1 激光扫描共焦显微镜 + 带荧光和 60X 物镜的 Arcturus 激光捕获显微切割系统 + 柯达成像站 + 富士BAS-5000荧光粉图像分析系统 + MCID 图像分析系统 (2) + AIS图像分析工作站 + StereoInvestigator 和 Neurolucida 定量细胞计数系统 + 3-D 细胞计数系统（Williams & Rakic） + Bioquant 细胞计数和成像系统 + 尼康光学显微镜，配有 Stereoinvestigator 软件 + 徕卡体视显微镜 + 蔡司 Axioskop 显微镜 (2) + Axioskop 数字显微工作站。 + 徕卡冷冻切片机这些复杂的成像系统使研究人员能够在三维空间中估计细胞、末端或突触的数量，并分析受体结合的密度和特定蛋白质的水平，从而使研究人员能够对细胞和分子神经病理学有前所未有的洞察力的精神障碍。