IMAGING CORE

成像核心

基本信息

批准号：
7720502
负责人：
GRAZYNA RAJKOWSKA
金额：
$ 10.22万
依托单位：
UNIVERSITY OF MISSISSIPPI MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-01 至 2009-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7720502
关键词：
3-Dimensional Animals Area Arts Autopsy Behavior Brain Brain region California Cell Count Chromosome Pairing Complement component C1s Computer Retrieval of Information on Scientific Projects Database Computer software Condition Confocal Microscopy D Cells Equipment Fluorescence Fluorescent Antibody Technique Funding Grant Head Histology Human Human Resources Image Image Analysis Imagery Immunohistochemistry Institution Instruction Laboratories Lasers Light Microscope Maintenance Measurement Medical center Mental disorders Methods Microscope Microscopic Microscopy Mississippi Molecular Morphology Neuroanatomy Neuroglia Neurons Neurosciences Research Paper Procedures Process Proteins Psychiatry Publishing Research Research Personnel Resources Sampling Scanning Slice Source Staining method Stains Supervision Synapses System Techniques Tissues Training United States National Institutes of Health Universities abstracting brain tissue cellular imaging cryostat density design digital experience insight investigator training laser capture microdissection neurochemistry neuropathology professor receptor binding

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As is the case for all areas of neuroscience research, the application of modern methods of cellular imaging are greatly accelerating advancements in the field. The center's Imaging Core gives investigators access to state-of-the-art, sophisticated microscopic equipment with advanced capabilities in the visualization, quantification and interpretation of neuronal systems at the cellular level. Dr. Grazyna Rajkowska, (Professor of Psychiatry and Human Behavior; Head of the Quantitative Neuroanatomy Laboratory), serves as Core Director of the CPN's Imaging Core. Dr. Rajkowska is a neuroanatomist with 20 years experience in quantitative image analysis of specific types of neurons and glial cells in human postmortem and animal brain tissues. She has served as Head of the Laboratory of Quantitative Neuroanatomy at UMC for the past 13 years and has published over 100 scientific papers and abstracts in the field of quantitative morphology. Dr. Rajkowska provides oversight of investigator training in the use of core equipment, the maintenance of core equipment, and the supervision of core personnel. In addition to the administrative oversight of the Imaging Core, Dr. Rajkowska instructs Center investigators and other UMC researchers in the general use of the microscopic imaging equipment and further offers highly specialized expertise in the technical parameters relevant to the design, application and interpretation of quantitative morphological and neurochemical studies. These technical parameters include tissue slicing and general histological staining of human postmortem and animal brain tissues, the application of 3-dimensional cell counting techniques to different types of staining procedures, the determination of optimal conditions for immunohistochemistry and guidance in the anatomical localization of the specific brain regions being studied. Dr. Jose Javier Miguel-Hidalgo (Assistant Professor of Psychiatry & Human Behavior, University of Mississippi Medical Center) is a neuroanatomist with 18 years experience in microscopy, histology and histochemical processing of brain samples in a variety of vertebrate species. Dr. Miguel-Hidalgo received formal training in confocal microscopy in 1992 at the University of California at Davis. CPN and UMC investigators often use confocal microscopy for double immunostaining with fluorescent antibodies. Dr. Miguel-Hidalgo also received instruction directly from Arcturus technical field trainers in 2005 and has subsequently trained several researchers at UMC using laser capture microdissection in their research. He further assists Dr. Rajkowska in determining the technical parameters (tissue sectioning, measurement, sampling, etc.) that would produce the best images for each investigator's particular application. The CPN's Imaging Core and UMC currently support a Imaging Facility providing shared use of the following imaging equipment: + Nikon C1 Laser Scanning Confocal Microscope + Arcturus Laser Capture Microdissection System with fluorescence and 60X objective + Kodak Imaging Station + Fuji BAS-5000 Phosphor Image Analysis System + MCID Image Analysis Systems (2) + AIS Image Analysis Workstation + StereoInvestigator and Neurolucida Quantitative Cell Counting System + 3-D Cell Counting System (Williams & Rakic) + Bioquant Cell Counting and Imaging System + Nikon Light Microscope, with stereoinvestigator software + Leica Stereomicroscope + Zeiss Axioskop Microscopes (2) + Axioskop Digital Photomicroscopy Workstation. + Leica Cryostat These sophisticated imaging systems give investigators the ability to estimate, in three-dimensional space, numbers of cells, terminals, or synapses and to analyze the density of receptor binding and the level of specific proteins, giving researchers unprecedented insight into the cellular and molecular neuropathology of mental disorders.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此，可以在其他清晰的条目中表示。列出的机构是对于中心，这不一定是调查员的机构。与神经科学研究的所有领域一样，现代细胞成像方法的应用在该领域的进步极大地加速了。该中心的成像核心使研究人员可以访问最先进的，精致的显微镜设备，并具有高级功能，可在细胞水平的神经元系统的可视化，量化和解释中进行高级功能。 Grazyna Rajkowska博士（精神病学和人类行为教授；定量神经解剖学实验室负责人）是CPN成像核心的核心主任。 Rajkowska博士是一种神经解剖学家，在人验尸和动物脑组织中特定类型的神经元和神经胶质细胞的定量图像分析方面具有20年的经验。在过去的13年中，她曾担任UMC定量神经解剖学实验室负责人，并在定量形态学领域发表了100多篇科学论文和摘要。 Rajkowska博士提供了调查员在使用核心设备，核心设备维护和核心人员的监督方面的监督。除了对成像核心的行政监督外，Rajkowska博士还指示中心调查人员和其他UMC研究人员的一般使用显微影像设备，并进一步提供了与设计，应用和解释有关定量的技术参数的高度专业化专业知识形态学和神经化学研究。这些技术参数包括组织切片和人类后脑组织和动物脑组织的一般组织学染色，将3维细胞计数技术应用于不同类型的染色程序，确定免疫组织化学的最佳条件和指导在特定特定的特定解剖学定位中正在研究大脑区域。 Jose Javier Miguel-Hidalgo博士（密西西比大学医学中心的精神病学与人类行为助理教授）是一位神经解剖学家，在各种脊椎动物中，在显微镜，组织学和组织化学处理方面具有18年的经验。 Miguel-Hidalgo博士于1992年在加利福尼亚大学戴维斯分校接受了共焦显微镜的正式培训。 CPN和UMC研究人员经常使用共聚焦显微镜与荧光抗体进行双重免疫染色。 Miguel-Hidalgo博士在2005年还直接从Arcturus技术野外培训师那里获得了指导，随后在其研究中使用激光捕获微分辨率在UMC培训了几位研究人员。他进一步协助Rajkowska博士确定技术参数（组织切片，测量，采样等），这些参数将为每个研究者的特定应用提供最佳图像。 CPN的成像核心和UMC当前支持成像设施，可提供以下成像设备的共同使用： +尼康C1激光扫描共聚焦显微镜 +带有荧光和60倍物镜的ARCTURUS激光捕获微分解系统 +柯达成像站 +富士BAS-5000磷光图分析系统 + MCID图像分析系统（2） + AIS图像分析工作站 +立体评估器和神经素定量细胞计数系统 + 3-D细胞计数系统（Williams＆Rakic） +生化细胞计数和成像系统 + Nikon Light显微镜，带有立体评估器软件 + Leica立体显微镜 + Zeiss Axioskop显微镜（2） + Axioskop数字显微镜工作站。 + Leica低温恒温器这些复杂的成像系统使研究人员能够在三维空间，细胞数量，末端或突触中估算能力，并分析受体结合的密度和特定蛋白质的水平，从而使研究人员对细胞和分子神经病理学的前所未有的见识精神障碍。