SC COBRE: PROTEIN SCIENCE CORE

SC COBRE：蛋白质科学核心

• 批准号: 8360389
• 负责人: YI-TE HSU
• 金额: $ 12.78万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-07-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360389
• 关键词: Achievement; Antibodies; Biological Assay; Cell Line; Centers of Research Excellence; Circular Dichroism; Consultations; Escherichia coli; Fatty acid glycerol esters; Funding; Generations; Generic Drugs; Grant; High Pressure Liquid Chromatography; Immunoprecipitation; Individual; Mammalian Cell; Mentors; National Center for Research Resources; Peptide Synthesis; Post-Translational Protein Processing; Principal Investigator; Proteins; Recombinant Proteins; Regulation; Research; Research Infrastructure; Research Personnel; Resources; Role; Science; Screening procedure; Services; Signal Transduction; Source; United States National Institutes of Health; Western Blotting; base; cost; design; instrument; interest; light scattering; lipid metabolism; medical specialties; physical property; protein E; protein structure function; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Protein Bioscience Core was created to assist COBRE lipidomics investigators in studying structures and functions of proteins involved in the regulation of lipid metabolism and signaling. Over the years, the Core has transitioned itself from a strictly consultation role to a more active role in servicing investigators in various aspects of protein science. This mainly included the generic service of expressing and purifying recombinant proteins from E. coli cultures. In addition, instruments such as dynamic light scattering and circular dichroism spectrometers were made available to researchers, together with in-depth tutoring, for the characterization of the physical properties of proteins. Recently, the Core started to offer more specialized services, including generation of stable mammalian cell lines expressing protein of interest, antibody design and purification, immunoprecipitation, protein crystal screening, peptide synthesis, protein modification, HPLC-based assays, and fat Western blotting. Because of the variety of services provided by the Protein Bioscience Core and the strong technical supporting base, they allow researchers who are not familiar with protein science, but are interested in studying protein structures and functions, to perform their experiments with greater ease. The aims of the Core are: 1) To produce proteins of interest for individual investigators that are needed for structural and functional characterization; 2) To offer a variety of protein-related specialty services to suit the needs of investigators; and 3) to enhance the understanding and capability of investigators in protein science through mentoring and consultation. Taken together, these aims should contribute to the scientific achievement of the targeted COBRE investigators.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 创建蛋白质生物科学核心是为了帮助毛脂科学研究者研究参与调节脂质代谢和信号传导的蛋白质的结构和功能。 多年来，核心已从严格的咨询角色转变为在为蛋白质科学各个方面服务研究人员的更积极作用。 这主要包括表达和净化大肠杆菌培养物重组蛋白的通用服务。 此外，为研究人员提供了诸如动态光散射和圆形二色性光谱仪之类的仪器，并深入辅导，以表征蛋白质的物理性质。最近，核心开始提供更多的专业服务，包括生成稳定的哺乳动物细胞系，表达感兴趣的蛋白质，抗体设计和纯化，免疫沉淀，蛋白质晶体筛选，肽合成，蛋白质修饰，基于HPLC的测定法和脂肪蛋白质印迹。由于蛋白质生物科学核心和强大的技术支持基础提供的多种服务，他们允许那些不熟悉蛋白质科学但有兴趣研究蛋白质结构和功能的研究人员，可以更轻松地进行实验。 核心的目的是：1）为结构和功能表征所需的单个研究人员产生感兴趣的蛋白质； 2）提供各种与蛋白质有关的专业服务，以满足调查人员的需求； 3）通过指导和咨询来增强蛋白质科学中研究人员的理解和能力。 综上所述，这些目标应为有针对性的鞋底研究人员的科学成就做出贡献。