TULANE COBRE: MOLECULAR, IMAGING AND ANALYTIC CORE

TULANE COBRE：分子、成像和分析核心

基本信息

批准号：
8360253
负责人：
Luis Gabriel Navar
金额：
$ 22.13万
依托单位：
TULANE UNIVERSITY OF LOUISIANA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2012-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8360253
关键词：
Agar Gel Electrophoresis Area Bathing Biology Cell Culture Techniques Cells Centers of Research Excellence Chemicals DNA Doctor of Philosophy Electrophoretic Mobility Shift Assay Equipment Fluorescence Funding Gene Targeting Genomics Genotype Glass Grant Histology Human Image Image Analysis Immunohistochemistry Incubators Laboratories Laboratory Research Medical Research Microscope Mus National Center for Research Resources New Brunswick Polyacrylamide Gel Electrophoresis Power Sources Principal Investigator Promoter Regions Proteins RNA Rattus Renal Hypertension Reporting Research Research Infrastructure Research Personnel Resources Reverse Transcriptase Polymerase Chain Reaction Robot Robotics Sampling Slide Source Specialist Staining method Stains System Systems Analysis Tail Time Tissues Training United States National Institutes of Health Water Western Blotting Work cost digital digital imaging microwave electromagnetic radiation molecular imaging ultraviolet

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Molecular, Imaging, and Analytical Core Laboratory consists of 3 rooms and 9 parts as follows: 1. Main Laboratory Workroom 1-1. DNA Workspace This space is dedicated for DNA work such as genotyping of gene-targeted mice and analyzing for promoter regions of target genes. A well-trained laboratory research technician will handle samples. He/She will extract genomic DNA from the snipped tails of gene-targeted mice and perform PCR. He/She will also set up agarose gel electrophoresis and report the final results to the corresponding investigators. Included major equipment are: Applied Biosystems GeneAmp PCR System, Fisher Scientific's dual chamber water bath, Sanyo's microwave, Fisher Scientific's power supply, Bio Rad's horizontal agarose gel-electrophoresis system, UVP's ultraviolet transilluminator, Fisher Scientific's portable centrifuge machine, and New Brunswick Scientific's incubator shaker. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Mike UPCHURCH, MS 1-2. Western Blot/Gel Shift Assay Area This space is dedicated for western blot analysis/gel shift assay. A well-trained laboratory research technician will handle samples. He/She will extract protein samplesfrom human/rats/mouse samples or cells provided by the investigators. He/She will also set up polyacrylamide gel electrophoresis and report the final results to the corresponding investigators. Included major equipments are: Licor's Odyssey system, Pharmacia Biotech's power supply and Novex's vertical PAGE system. Postdoctoral Research Fellow: Kayoko MIYATA, PhD Laboratory Research Technician: Salem I. ELKHAYAT, MS 1-3. Shared Open Space This open space is shared as a pipetting station. Included major equipment are: Next Advance Inc.'s Bullet Blender BLUE, Fisher Scientific's accuSpin Micro17R refrigerated microcentrifuge, Denver Instrument¿s high quality scale, Thermolyne's platform shaker and Fisher Scientific's stirring hotplate. 1-4. Histology/Immunohistochemistry Workspace This space is dedicated for histology/ immunohistochemistry works. A well-trained laboratory research technician will handle samples. He/She will set up slide glasses of human/rats/mouse samples provided by the investigators. He/She will also perform histology and/or immunohistochemistry staining and return the stained slides to the corresponding investigators. Included major equipment are: Dako Cytomation's Autostainer for immunohistochemistry robot, Fisher Scientific's chemical hume hood for straining, Fisher Scientific's slide oven. Postdoctoral Research Fellow: Masumi KAMIYAMA, PhD Laboratory Research Technician: Salem I. ELKHAYAT, MS 1-6. RNA Workspace This space is dedicated for RNA work such like RNA extraction and real-time RT-PCR.A well-trained laboratory research technician will handle samples. He/She will extract total RNA samples from human/rats/mouse samples or cells provided by the investigators using Qiagen's robotic system. He/She will also set up real-time RT-PCR and report the final results to the corresponding investigators. Included major equipments are: Thermo Scientific's NanoDrop 2000, Qiagen's BioRobot EZ1, Amersham Biosciences' GeneQuant spectrometer, Fisher Scientific's portable centrifuge machine and Fisher Scientific's tissue homogenizer. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Mike UPCHURCH, MS 1-7. Analytical Equipment Station This space is dedicated for analytical equipments. Major equipment are: Stratagene's real-time PCR machines (MX3000P and MX3005P) and Alphainotech's densitometric machine. 2. Cell Culture Workroom A separated room is dedicated for cell cultures. Included major equipment are: Nexcelom Cellometer's Automated Cell Counter, Labconco's purifier class II biosafety cabinet Heraeus's cell incubator, Fisher Scientific's inverted microscope and Eppendorf's bench-top refrigerated centrifuge machine. Postdoctoral Research Fellow: Ryo SATO, PhD Laboratory Research Technician: G. Mike UPCHURCH, MS 3. Microscope Workroom A separate room is dedicated for microscopes and image analysis. Major equipment are: EVOS fl digital inverted microscope, Olympus's (general) microscope equipped to real-time digital image capturing system, Olympus's fluorescence-ready microscope equipped to real-time digital image capturing system, and Image-Pro plusimage handling/analyzing system. Postdoctoral Research Fellow: Masumi KAMIYAMA, PhD Medical Research Specialist: Akemi SATO, MS Laboratory Research Technician: Salem I. ELKHAYAT, MS

该子项目是利用资源的众多研究子项目之一由 NIH/NCRR 资助的中心拨款提供该子项目的主要支持。并且子项目的首席研究员可能是由其他来源提供的，包括其他 NIH 来源的子项目可能列出的总成本。代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。分子、成像和分析核心实验室由3个房间和9个部分组成，如下： 1.主实验室工作室 1-1. DNA 工作区该空间专门用于 DNA 工作，例如基因靶向小鼠的基因分型和目标基因启动子区域的分析。训练有素的实验室研究技术人员将从剪断的基因靶向小鼠尾部提取基因组 DNA。他/她还将设置琼脂糖凝胶电泳并向研究人员报告最终结果，包括：Applied Biosystems GeneAmp PCR 系统、Fisher Scientific 的双室水浴、Sanyo 的微波炉、 Fisher Scientific 的电源、Bio Rad 的水平琼脂糖凝胶电泳系统、UVP 的紫外线透照仪、Fisher Scientific 的便携式离心机和 New Brunswick Scientific 的培养箱摇床。博士后研究员：Ryo SATO，博士实验室研究技术员：G. Mike UPCHURCH, MS 1-2. 蛋白质印迹/凝胶转移测定区域该空间专门用于蛋白质印迹分析/凝胶转移测定，他/她将从研究人员提供的人类/大鼠/小鼠样本或细胞中提取蛋白质样本。建立聚丙烯酰胺凝胶电泳并向相应的研究者报告最终结果，包括的主要设备有：Licor的Odyssey系统、Pharmacia Biotech的电源和Novex的垂直PAGE系统。博士后研究员：宫田佳代子博士实验室研究技术员：Salem I. ELKHAYAT，MS 1-3. 共享开放空间该开放空间作为移液站共享，包括的主要设备包括：Next Advance Inc. 的 Bullet Blender BLUE、Fisher Scientific 的 AccuSpin Micro17R 冷冻微量离心机、Denver Instrument¿的高品质天平、Thermolyne 的平台摇床和 Fisher Scientific 的搅拌电炉。 1-4. 组织学/免疫组织化学工作区该空间专门用于组织学/免疫组织化学工作。训练有素的实验室研究技术人员将处理研究人员提供的人类/大鼠/小鼠样本的载玻片，并且/她还将进行组织学研究。或免疫组织化学染色并将染色的载玻片返回给相应的研究人员，包括的主要设备包括：用于免疫组织化学机器人的 Dako Cytomation 的自动染色机、Fisher Scientific 的化学休谟罩。用于过滤，Fisher Scientific 的滑动烘箱。博士后研究员：Masumi KAMIYAMA 博士实验室研究技术员：Salem I. ELKHAYAT，MS 1-6.RNA工作区该空间专门用于 RNA 提取和实时 RT-PCR 等 RNA 工作。训练有素的实验室研究技术人员将从人/大鼠/小鼠样本或细胞提供的样本中提取总 RNA 样本。研究人员使用Qiagen的机器人系统，他/她还将设置实时RT-PCR并向相应的研究人员报告最终结果，包括：Thermo Scientific的NanoDrop 2000、Qiagen的BioRobot。 EZ1、Amersham Biosciences 的 GeneQuant 光谱仪、Fisher Scientific 的便携式离心机和 Fisher Scientific 的组织匀浆器。博士后研究员：Ryo SATO，博士实验室研究技术员：G. Mike UPCHURCH, MS 1-7. 分析设备站该空间专用于分析设备，主要设备有：Stratagene 的实时 PCR 仪（MX3000P 和 MX3005P）和 Alphainotech 的光密度仪。 2.细胞培养工作室一个独立的房间专门用于细胞培养，主要设备包括：Nexcelom Cellometer 的自动细胞计数器、Labconco 的 II 级生物安全柜、Heraeus 的细胞培养箱、Fisher Scientific 的倒置显微镜和 Eppendorf 的台式冷冻离心机。博士后研究员：Ryo SATO，博士实验室研究技术员：G. Mike UPCHURCH, MS 3.显微镜工作室一个单独的房间专门用于显微镜和图像分析，主要设备有：EVOS fl数字倒置显微镜、配备实时数字图像捕获系统的奥林巴斯（普通）显微镜、配备实时数字图像捕获系统的奥林巴斯荧光显微镜。、Image-Pro plus 图像处理/分析系统。博士后研究员：Masumi KAMIYAMA 博士医学研究专家：佐藤明美，MS 实验室研究技术员：Salem I. ELKHAYAT，MS