MOLECULAR BIOLOGY CORE

分子生物学核心

• 批准号: 8360505
• 负责人: MARK C AUSTIN
• 金额: $ 13.69万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360505
• 关键词: Biological; Brain; Color; Computer software; Consultations; DNA; Detection; Documentation; Enzymes; Faculty; Funding; Gel; Gene Expression Regulation; Grant; Image Analysis; Imagery; Individual; Microscope; Molecular; Molecular Biology; National Center for Research Resources; Neuraxis; Neuronal Plasticity; Neurons; Neuropeptides; Neurosciences; Neurotransmitters; Principal Investigator; Process; RNA; Research; Research Infrastructure; Research Personnel; Resources; Role; Sampling; Scintillation Counter; Source; Staging; Structure; Synaptic Transmission; System; Systems Analysis; Techniques; Thinking; Time; Training; United States National Institutes of Health; austin; base; cost; cryostat; digital; interest; laser capture microdissection; receptor; relating to nervous system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Over the past decade, the application of molecular biological techniques to the study of the central nervous system has revolutionized our thinking about synaptic transmission, neuronal plasticity, and neuronal integration, as well as the molecular basis of psychiatric illnesses. The center's Molecular Biology Core emphasizes the application of biotechnologically advanced molecular techniques to study the expression, structure, function, and localization of a variety of neural substrates in the brain. The Molecular Biology Core provides training, consultation, and technical assistance in molecular biological approaches that allow individual researchers to study various molecular endpoints, regulatory mechanisms, as well as the role of transcriptional and translational processes in regulating and controlling the expression of the various neural substrates of interest. Dr. Mark Austin, Core Leader of the Molecular Biology Core, is a molecular neuropharmacologist with expertise in utilizing various molecular biological approaches to study the regulation of gene expression of neurotransmitter enzymes, receptors, transporters and neuropeptides. The Molecular Biology Core provides shared-access to the following amplification, visualization, detection and analysis systems: + Arcturus Veritas Laser Capture Microdissection System + MyiQ Single-Color Real-Time PCR Detection System with SW/Beacon Designer Cycler IQ software + BioRad Gel Documentation System + NanoDrop ND-1000A UV-Vis Spectrophotometer (1-2 ¿l samples) + MCID Elite Image analysis system with Zeiss Axioskop Microscope and Ludl motorized stage + Veritas Microplate Luminometer + Bio-Rad Thermal cycler + BioRad Real-Time PCR Machine + Zeiss Axioskop Digital Photomicroscopy Workstation + GeneQuant RNA/DNA calculator + Packard Scintillation Counter + Leica Cryostat The Molecular Biology Core serves as a central resource that enables, stimulates and facilitates the use of molecular biological approaches and further enhances the methodological diversity of Center Investigators as well as other departmental faculty.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在过去的十年中，分子生物学技术在中枢神经系统研究中的应用彻底改变了我们对突触传播，神经元可塑性和神经元整合的思考，以及精神病的分子基础。该中心的分子生物学核心强调了生物技术先进的分子技术在研究大脑中各种神经元底物的表达，结构，功能和定位。分子生物学核心在分子生物学方法中提供了培训，咨询和技术援助，使单个研究人员能够研究各种分子终点，调节机制，以及转录和翻译过程在确定和控制各种感兴趣神经底物的表达中的作用。 分子生物学核心的核心领导者马克·奥斯汀（Mark Austin）博士是一位分子神经药物学家，具有使用各种分子生物学方法来研究神经递质酶，受体，转运蛋白和神经肽的基因表达的调节。 分子生物学核心为以下放大，可视化，检测和分析系统提供了共享的访问： + Arcturus Veritas激光捕获微分解系统 +带有SW/Beacon Designer Cycler IQ软件的MyIQ单色实时PCR检测系统 + Biorad凝胶文档系统 + Nanodrop ND-1000A UV-VIS分光光度计（1-2»样品） + Zeiss Axioskop显微镜和Ludl机动阶段的MCID精英图像分析系统 + Veritas微板发光仪 + Bio-Rad热循环器 + Biorad实时PCR机器 + Zeiss Axioskop数字显微镜工作站 + Genequant RNA/DNA计算器 + Packard闪烁柜台 + Leica低温恒温器 分子生物学核心是一种核心资源，可促进，刺激和促进分子生物学方法的使用，并进一步增强中心研究人员以及其他部门的方法论多样性。