MOLECULAR BIOLOGY CORE

分子生物学核心

• 批准号: 7720503
• 负责人: MARK C AUSTIN
• 金额: $ 8.73万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720503
• 关键词: Biological; Brain; Color; Computer Retrieval of Information on Scientific Projects Database; Computer software; Consultations; Detection; Documentation; End Point; Enzymes; Faculty; Funding; Gel; Gene Expression Regulation; Grant; Image Analysis; Imagery; Individual; Institution; Microscope; Molecular; Molecular Biology; Neuraxis; Neuronal Plasticity; Neurons; Neuropeptides; Neurotransmitters; Polymerase Chain Reaction; Process; Research; Research Personnel; Resources; Role; Sampling; Scintillation Counter; Source; Staging; Structure; Synaptic Transmission; System; Systems Analysis; Techniques; Thinking; Time; Training; United States National Institutes of Health; austin; base; cryostat; digital; interest; laser capture microdissection; receptor; relating to nervous system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Over the past decade, the application of molecular biological techniques to the study of the central nervous system has revolutionized our thinking about synaptic transmission, neuronal plasticity, and neuronal integration, as well as the molecular basis of psychiatric illnesses. The center's Molecular Biology Core emphasizes the application of biotechnologically advanced molecular techniques to study the expression, structure, function, and localization of a variety of neural substrates in the brain. The Molecular Biology Core provides training, consultation, and technical assistance in molecular biological approaches that allow individual researchers to study various molecular endpoints, regulatory mechanisms, as well as the role of transcriptional and translational processes in regulating and controlling the expression of the various neural substrates of interest. Dr. Mark Austin, Core Leader of the Molecular Biology Core, is a molecular neuropharmacologist with expertise in utilizing various molecular biological approaches to study the regulation of gene expression of neurotransmitter enzymes, receptors, transporters and neuropeptides. The Molecular Biology Core provides shared-access to the following amplification, visualization, detection and analysis systems: + Arcturus Veritas Laser Capture Microdissection System + MyiQ Single-Color Real-Time PCR Detection System with SW/Beacon Designer Cycler IQ software + BioRad Gel Documentation System + NanoDrop ND-1000A UV-Vis Spectrophotometer (1-2 ¿l samples) + MCID Elite Image analysis system with Zeiss Axioskop Microscope and Ludl motorized stage + Veritas Microplate Luminometer + Bio-Rad Thermal cycler + BioRad Real-Time PCR Machine + Zeiss Axioskop Digital Photomicroscopy Workstation + GeneQuant RNA/DNA calculator + Packard Scintillation Counter + Leica Cryostat The Molecular Biology Core serves as a central resource that enables, stimulates and facilitates the use of molecular biological approaches and further enhances the methodological diversity of Center Investigators as well as other departmental faculty.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以出现在其他 CRISP 条目中 列出的机构是。 对于中心来说，它不一定是研究者的机构。 在过去的十年中，分子生物学技术在中枢神经系统研究中的应用彻底改变了我们对突触传递、神经元可塑性和神经元整合以及精神疾病的分子基础的思考。应用生物技术先进的分子技术来研究大脑中各种神经底物的表达、结构、功能和定位。分子生物学核心提供分子生物学方法的培训、咨询和技术援助，使个体研究人员能够使用。研究各种分子终点、调节机制以及转录和翻译过程在调节和控制各种感兴趣的神经底物表达中的作用。 马克·奥斯汀博士是分子生物学核心的核心领导者，是一位分子神经药理学家，拥有利用各种分子生物学方法来研究神经递质酶、受体、转运蛋白和神经肽的基因表达调节的专业知识。 分子生物学核心提供对以下放大、可视化、检测和分析系统的共享访问： + Arcturus Veritas 激光捕获显微切割系统 + MyiQ 单色实时 PCR 检测系统，配有 SW/Beacon Designer Cycler IQ 软件 + BioRad 凝胶记录系统 + NanoDrop ND-1000A 紫外-可见分光光度计（1-2 ¿l 样品） + MCID Elite 图像分析系统，配备 Zeiss Axioskop 显微镜和 Ludl 电动载物台 + Veritas 微孔板发光计 + Bio-Rad 热循环仪 + BioRad 实时荧光定量 PCR 机 + Zeiss Axioskop 数字显微工作站 + GeneQuant RNA/DNA 计算器 +帕卡德闪烁计数器 + 徕卡冷冻切片机 分子生物学核心作为一个中心资源，能够实现、刺激和促进分子生物学方法的使用，并进一步增强中心研究人员以及其他院系教师的方法多样性。