ORAL DISTRIBUTION OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS IN OLD WORLD MONKEYS

旧世界猴群中放线菌共生菌的口腔分布

• 批准号: 8358013
• 负责人: DANIEL H FINE
• 金额: $ 5.4万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358013
• 关键词: Antibiotic Resistance; Bacteria; Biological Assay; Cell membrane; Cercopithecidae; Dental Plaque; Disinfection; Epithelial Cells; Epithelium; Funding; Grant; Human; In Vitro; Knock-out; Ligands; Local Anti-Infective Agents; Mediating; Membrane Proteins; Modeling; Monkeys; National Center for Research Resources; New England; Oral; Oral cavity; Pattern; Periodontitis; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Role; Solutions; Source; Swab; Teenagers; United States National Institutes of Health; base; cost; human disease; nonhuman primate; novel; oral bacteria; preadolescence; receptor; resistant strain

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Aggregatibacter actinomycetemcomitans (Aa) is a gram negative capnophilic oral bacteria and the putative cause of Localized Aggressive Periodontitis (LAP) in preteens and teenagers. It has been shown in vitro to colonize buccal epithelial cells of humans and old world monkeys exclusively. This attachment is mediated by bacterial surface proteins, aae and apiA which are receptors which attach to ligands on the buccal epithelial cell membrane. Further, the buccal epithelium appears to be a primary reservoir of Aa in humans. The purpose of this study is to determine the earliest patterns of oral colonization of Aa in the primate mouth and determine the roles of apiA and aae in the pattern of colonization. Monkeys are screened with oral swabs and dental plaque scraping for presence of Aa bacteria and the strain of bacteria identified with novel PCR assays. Additionally, a smaller group of monkeys is inoculated with Aa bacteria following disinfection of the oral cavity with antiseptic solution. Inoculation of various strains including knockout and antibiotic resistant strains will help to determine the host range for Aa bacteria and the use of a nonhuman primate model fo the human disease.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 放线菌聚集菌 (Aa) 是一种革兰氏阴性嗜二氧化碳口腔细菌，是青少年局部侵袭性牙周炎 (LAP) 的假定原因。 体外研究表明，它只能定植于人类和旧大陆猴的颊上皮细胞。 这种附着是由细菌表面蛋白 aae 和 apiA 介导的，它们是附着在颊上皮细胞膜上的配体上的受体。 此外，颊上皮似乎是人类 Aa 的主要储存库。 本研究的目的是确定 Aa 在灵长类动物口腔中最早的口腔定植模式，并确定 apiA 和 aae 在定植模式中的作用。 通过口腔拭子和牙菌斑刮片对猴子进行筛查，以确定是否存在 Aa 细菌以及通过新型 PCR 检测方法鉴定出的细菌菌株。 此外，一小群猴子在用消毒液消毒口腔后接种了 Aa 细菌。 接种包括敲除菌株和抗生素抗性菌株在内的各种菌株将有助于确定 Aa 细菌的宿主范围以及使用非人类灵长类动物模型来治疗人类疾病。