MONKEY CORTICAL CONNECTIONS DATABASE

猴子皮质连接数据库

• 批准号: 8357302
• 负责人: Edward G Jones
• 金额: $ 2.52万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357302
• 关键词: Adult; Architecture; Archives; Area; Atlases; Back; Brain; Brain imaging; California; Cerebral cortex; Cerebrum; Clinical Data; Cognition; Data; Data Set; Databases; Disabled Persons; Disease; Esthesia; Functional disorder; Funding; Grant; Image; Individual; Knowledge; Life; Macaca mulatta; Microscopic; Monkeys; Names; National Center for Research Resources; Neurologic; Neurosciences; Perception; Primates; Principal Investigator; Publications; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Retrieval; Series; Slide; Source; Staining method; Stains; Structure; Tracer; United States National Institutes of Health; Visual; base; cost; digital; handicapping condition; high standard; image warping; interoperability; neuropsychiatry; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Knowledge of cerebral cortical connectivity in primates is essential for understanding higher brain mechanisms of sensation, perception and cognition and their disorders but connection tracing studies are increasingly uncommon, older studies are accessible only through publications which represent an earlier investigator's interpretation of the data, and access to the original data for re-interpretation is commonly unavailable. Retrieval of archives of experimental material and conversion to an accessible digital form presents considerable logistical and other handicaps. We seek to develop a comprehensive and ongoing archive of material demonstrating cortical and subcortical connectivity in rhesus monkey brains, in which all the histological material from individual experiments is available in its digital form, with a suitable search engine providing open access to all investigators. In this way, original experimental data can be annotated and re-interpreted as newer parcellations of cortical and subcortical structures are made on the basis of accumulating functional and clinical data. The current proposal builds on expertise in constructing high resolution microscopic atlases of primate brain architecture and will create a searchable database of connections of the cerebral cortex in the adult rhesus monkey. A pipeline will be established in which targeted cortical areas in a series of monkeys will be injected with a tracer transported both retrogradely and anterogradely. Digital data about each individual brain will be collected by means of: structural mri; serial imaging of the sectioned blockface as each 40¿m section is removed during sectioning of the brain; imaging at high resolution of alternate sections through the brain stained for the transported tracer or stained with the nissl stain; warping images of the sections back onto the blockface images of the brain and mris to provide a 3d volumetric dataset for each brain. The searchable database into which the individual datasets will be stored will incorporate a capacity to annotate the dataset from each experimental brain in relation to a standard high resolution digital atlas of the rhesus monkey brain, a capacity for making queries about connections by name or by visual inspection of injected regions or of likely sources of afferents and targets of efferents, a capacity for interoperability with databases of other relevant neuroscience information. The database will also permit incorporation of datasets of connectivity drawn from material in publications and/or existing slide. The result will be a living archive to which data essential for the understanding of the pathophysiology of neurological and neuropsychiatric disorders can continue to be added and queried.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 对主要中脑皮质连通性的了解对于理解更高的大脑感觉，感知和认知及其疾病的较高大脑机制，但是连接追踪研究越来越不常见，较旧的研究只能通过代表早期研究者对数据的解释，以及对重新解释的原始数据的访问的访问而访问的出版物，通常是不可生存的。检索实验材料档案和转换为可访问的数字形式的档案呈现出相当大的后勤和其他障碍。我们试图制定一个全面且持续的档案，这些材料展示了恒河猴猴子的皮质和皮层连通性，其中所有实验的所有组织学材料都以数字形式获得，并提供了适当的搜索引擎，可为所有研究人员提供开放式访问权限。这样，可以将原始的实验数据注释并重新解释，因为根据积累功能和临床数据来制作皮质和皮层结构的较新拟化。当前的提案建立在构建原发性大脑体系结构高分辨率微观图书馆的专家基础上，并将在成人恒河猴中创建一个可搜索的大脑皮层连接数据库。将建立一条管道，其中一系列猴子中的靶向皮质区域将被注射逆行和抗抗拉的示踪剂。将通过以下方式收集有关每个大脑的数字数据：结构MRI；在大脑切片过程中除去每个40“截面时，截面的区块的串行成像；在高分辨率下通过大脑染色的示踪剂或用NISSL染色染色的大脑成像；部分的翘曲图像回到了大脑和MRI的区块脸图像上，为每个大脑提供3D体积数据集。将存储各个数据集中的可搜索数据库将结合与标准的恒河猴大脑的标准高分辨率数字地图相关的数据集，以通过名称或可视化区域的视觉检查或其他可能的能力来源的能力和其他有效性的范围来求解恒河猴大脑的标准数据集，以询问连接的能力。该数据库还将允许合并出版物和/或现有幻灯片中材料中绘制的连接性数据集。结果将是一个活生生的档案，可以继续添加和询问数据对理解神经和神经精神疾病的病理生物生物生物生物生物生物生物生物生物的必不可少的数据。