INTRODUCING STABLE INFERTILITY BY RNA INTERFERENCE

通过 RNA 干扰引入稳定的不孕症

• 批准号: 8357818
• 负责人: Sergio R Ojeda
• 金额: $ 4.36万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357818
• 关键词: Blood; Brain; Canis familiaris; Cells; Epitopes; Felis catus; Funding; Gene Expression; Goals; Grant; Home environment; Hypothalamic structure; Infertility; Iowa; National Center for Research Resources; Oregon; Ovary; Peptides; Phase; Population; Primates; Principal Investigator; RNA Interference; Rattus; Research; Research Infrastructure; Resources; Source; Stream; System; Tropism; United States National Institutes of Health; Universities; Viral; base; cost; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The overall goal of the present study is to develop a system for longlasting inhibition of gene expression in the brain or ovary that can be used to cause permanent infertility in dogs and cats. This application is a collaborative effort between the University of Iowa (UI) and the Oregon National Primate Research Center (ONPRC). We have performed several experiments aimed at achieving the objectives of the original application. These experiments involve the use of a bio-panning strategy to identify peptide epitopes that can be delivered via the blood stream and specifically home to cells in either the hypothalamus or the ovary of the rat to deliver RNAi. After five rounds of bio-panning, we selected an epitope that specifically home to the hypothalamus, ovary or both, and that can therefore be used for the next phase of the study: re-directing viral tropism of AAV based on these selected epitopes to deliver RNA interference to those particular cell populations.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 本研究的总体目的是开发一个系统，以持续抑制大脑或卵巢中基因表达的持续抑制，该系统可用于引起狗和猫的永久性不育症。该应用程序是爱荷华大学（UI）和俄勒冈州国家灵长类动物研究中心（ONPRC）之间的合作努力。 我们进行了几项旨在实现原始应用程序目标的实验。这些实验涉及使用生物剥离策略来鉴定可以通过血液流传递的肽表位，并在下丘脑或大鼠的卵巢中特别是细胞的家用，以提供RNAi。经过五轮生物铺设后，我们选择了一个特别是下丘脑，卵巢或两者兼而有之的表位，因此可以用于研究的下一阶段：基于这些选定的表现，将AAV的病毒性向热态重新指导，以将RNA干扰输送到这些特定细胞种群中。