CTL EXCLUSION FROM LYMPHOID FOLLICLES AND LENTIVIRUS IMMUNE EVASION

CTL 从淋巴滤泡中排除和慢病毒免疫逃避

• 批准号: 8358245
• 负责人: PAMELA J SKINNER
• 金额: $ 4.29万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358245
• 关键词: Animals; Area; Blood; CD8B1 gene; Cell Line; Cells; Cytotoxic T-Lymphocytes; Exclusion; Funding; Grant; HIV; Immune; Immunology; Lymph Node Part; Lymphoid Follicle; Lymphoid Tissue; Macaca mulatta; National Center for Research Resources; Pilot Projects; Population; Primates; Principal Investigator; Process; Proliferating; Research; Research Infrastructure; Resources; Role; Services; Site; Source; Subfamily lentivirinae; T-Lymphocyte; United States National Institutes of Health; Virus; Virus Diseases; Wisconsin; cost; in vivo; killings; lymph nodes

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To investigate potential mechanisms underlying the distribution of virus-specific CTL and virus-producing cells in lymphoid tissues of chronically SIVmac239-infected rhesus macaques and determine whether lentivirus-specific CTL activity is restricted to the extrafollicular compartment of lymphoid tissues in vivo. During HIV (human immunodeficiency virus) infection, an enormous number of cytotoxic T cells are elicited. The role of this cell population is to eliminate virally infected cells. However in spite the large number of HIV specific cytotoxic T cells in the blood, the virus proliferates very efficiently in some part of the lymph nodes. We hypothesize that certain parts of the lymph nodes are immune privileged and cytotoxic T lymphocytes (CTL) are unable to enter to these sites. As a consequence, CTL can kill infected cells in some areas of the lymph nodes, but not others. In this pilot study we are going to infect three animals to determine the distribution of virus-specific CTL and virus-producing cells in lymphoid tissues of chronically SIVmac239-infected rhesus macaques and determine whether lentivirus-specific CTL activity is restricted to the extrafollicular compartment of lymphoid tissues in vivo. PROGRESS: We have established a B-LCL cell line from a selected animal. We are in the process of establishing CD8+ T cell lines. This research used WNPRC Animal Services, CPI and Immunology Services. PUBLICATIONS: None.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 目的：研究慢性SIVMAC239感染的恒河猕猴的淋巴组织中病毒特异性CTL和产生病毒细胞分布的潜在机制，并确定菌根特异性的CTL活性是否仅限于淋巴样组织的炎外膜外群。 在HIV（人类免疫缺陷病毒）感染期间，引起了大量的细胞毒性T细胞。该细胞种群的作用是消除病毒感染的细胞。然而，尽管血液中大量的HIV特异性细胞毒性T细胞，但该病毒在淋巴结的某些部分中非常有效地增殖。我们假设淋巴结的某些部位是免疫特权的，细胞毒性T淋巴细胞（CTL）无法进入这些部位。结果，CTL可以在淋巴结的某些区域杀死受感染的细胞，而不是其他区域。在这项试验研究中，我们将感染三只动物，以确定病毒特异性CTL和病毒产生细胞在慢性SIVMAC239感染的恒河猴的淋巴组织中的分布，并确定肺炎病毒特异性的CTL活性是否限制在淋巴机外裂解的叶膜组织中。 进步： 我们已经从选定的动物建立了B-LCL细胞系。我们正在建立CD8+ T细胞系。 这项研究使用了WNPRC动物服务，CPI和免疫学服务。 出版物： 没有任何。