IN SITU SIV-SPECIFIC CD4 T CELL ANALYSIS DURING ACUTE SIV INFECTION

急性 SIV 感染期间 SIV 特异性 CD4 T 细胞原位分析

基本信息

批准号：
7958804
负责人：
PAMELA J SKINNER
金额：
$ 19.66万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2010-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7958804
关键词：
Acute Antigens Biology CD4 Positive T Lymphocytes Collaborations Computer Retrieval of Information on Scientific Projects Database Funding Grant In Situ Institution Knowledge Macaca Methodology Methods Minnesota Mission Primates Reagent Research Research Personnel Resources SIV Scientist Source Spleen Staining method Stains T-Cell Receptor Tissues Transgenic Mice United States National Institutes of Health Universities Virus Diseases Wisconsin human disease

Acute Antigens Biology CD4 Positive T Lymphocytes Collaborations Computer Retrieval of Information on Scientific Projects Database Funding Grant In Situ Institution Knowledge Macaca Methodology Methods Minnesota Mission Primates Reagent Research Research Personnel Resources SIV Scientist Source Spleen Staining method Stains T-Cell Receptor Tissues Transgenic Mice United States National Institutes of Health Universities Virus Diseases Wisconsin human disease

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To target the Wisconsin National Primate Research Center's mission to develop treatments for human disease, generate knowledge of primate biology, and to facilitate the progress of research by providing expertise and resources to external scientists, we are developing a methodology that we will use to determine the abundance and in situ localization of CD4 T cells specific to simian immunodeficiency virus (SIV) in tissues during SIV infection. Results: Although we have not yet received the pilot grant money from the WNPRC for the project, we have nonetheless started to make some progress. In collaboration with Marc Jenkins at the University of Minnesota we are developing methods to stain antigen specific CD4 T cells in spleen tissues from T cell receptor transgenic mice and Nancy Wilson at the WNPRC is making progress developing class II reagents for use in SIV-infected macaques.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。目的：针对威斯康星州国家灵长类动物研究中心（National Primate Research Center）的使命，旨在通过向外部科学家提供专业知识和资源来开发人类疾病的治疗方法，产生灵长类动物生物学知识，并促进研究的进步，我们正在开发一种方法，我们将使用该方法来确定与Simian Immundicficitige virus（SIV）中的CD4 T细胞的丰度和原位定位的SIV（SIV）在SIV中。结果：尽管我们尚未从WNPRC获得该项目的飞行员赠款，但我们还是开始取得了一些进展。与明尼苏达大学的Marc Jenkins合作，我们正在开发从T细胞受体转基因小鼠和WNPRC的Nancy Wilson的脾脏组织中染色抗原特异性CD4 T细胞的方法。