VECTOR-BORNE DISEASES CORE

媒介传播疾病核心

• 批准号: 8358066
• 负责人: MARIO TOMAS PHILIPP
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358066
• 关键词: Adult; Affect; Anopheles Genus; Black-legged Tick; Blood; Blood capillaries; Borrelia burgdorferi; Collaborations; Development; Ensure; Female; Funding; Future; Grant; Health Sciences; Human; Immersion Investigative Technique; Immune response; Infection; Larva; Liquid substance; Lyme Disease; Macaca mulatta; Modeling; Molting; Mus; National Center for Research Resources; Nymph; Order Spirochaetales; Plasmodium cynomolgi; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Source; Specimen; Staging; Techniques; Texas; Ticks; Time; United States National Institutes of Health; Universities; Virulence; Vivax Malaria; capillary; cost; egg; feeding; research study; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The VBD core has continued to maintain an Anopheles stephensi colony, which is used to transmit Plasmodium cynomolgi, a model of human vivax malaria, to rhesus macaques. The tick colony has continued to be instrumental in enabling our division to do research in Lyme borreliosis using the natural mode of infection. The technique of capillary feeding of nymphal ticks, which we have available, allows us to infect ticks with spirochetal clonal isolates. This is often essential to ensure defined host-responses to infection. We also are able to infect larval ticks by immersion in tissue-culture fluid that contains spirochetes. The tick section of the core has currently available numerous specimens of all of the developmental stages of Ixodes scapularis. Larvae, nymphs, and adults are stored at 4¿C in a staggered fashion. Therefore, we usually have all of the stages available at most times throughout the year. This includes 5-10 jars of larvae (with about 1000 larvae each) and several hundred uninfected nymphs for experimental needs as they arise. We have continued our collaboration with the University of Texas Health Sciences Center, Houston, TX, on the identification of virulence determinants of B. burgdorferi the affect infectivity of spirochetes to ticks, and to mice via ticks. This year 81 adult females were collected and were blood fed. A total of 51 of those fed successfully, and 36 of those laid eggs. The number of larvae that fed and were collected were 1982, and 1535 of those successfully molted into nymphs to be used in future experiments. A total of 497 nymphs were capillary-fed successfully. Those ticks were used in 3 large experiments. The remainder of the nymphs will be used in the current year.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 VBD核心继续维持一个按需卵巢菌落，该菌落用于将人类Vivax疟疾模型的疟原虫cynomolgi传播到恒河猴。壁虱菌落继续使用自然感染模式来使我们的划分能够在莱姆毛毛虫病中进行研究。我们提供的若虫壁虱的毛细血管喂养技术使我们能够用螺旋齿克隆分离株感染tick虫。这通常对于确保定义的宿主响应至关重要。我们还能够通过浸入含有螺旋体的组织培养液中感染幼体s虫。核心的刻度部分目前已获得ixodes肩cap骨的所有发育阶段的众多标本。幼虫，若虫和成年人以交错的方式存放在4 c上。因此，我们通常在全年的大部分时间都有所有阶段。这包括5-10罐幼虫（每个幼虫约1000个）和几百个未感染的若虫，以满足实验需求。我们一直在与德克萨斯州休斯顿市德克萨斯大学健康科学中心合作，以确定病毒确定B. burgdorferi的识别，即螺旋体对tick虫的影响以及通过tick的小鼠的影响。今年收集了81名成年女性并获得血液喂养。成功喂养的人中，共有51个，其中36个产卵。饲料和收集的幼虫数量为1982年，其中1535个成功划分为若虫的幼虫数量在以后的实验中使用。总共497个若虫成功地喂养了毛细血管喂养。这些壁虱用于3个大实验。其余的若虫将在今年使用。