CHARACTERIZATION OF A MORAXELLA SPECIES THAT CAUSES EPSTAXIS IN MACAQUES

导致猕猴腹泻的莫拉氏菌属物种的特征

• 批准号: 8173026
• 负责人: MARIO TOMAS PHILIPP
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173026
• 关键词: Animals; Bacteria; Biochemical; Cells; Communicable Diseases; Computer Retrieval of Information on Scientific Projects Database; Disease; Epistaxis; Funding; Gram' s stain; Gram-Negative Aerobic Bacteria; Grant; Human; Institution; Macaca; Macaca mulatta; Medical Research; Moraxella; Moraxella (Branhamella) catarrhalis; Morphology; Nasopharynx; Nose; Primates; Research; Research Institute; Research Personnel; Resources; Respiratory System; Respiratory tract structure; Source; Surveys; Syndrome; Testing; United States National Institutes of Health; base; member; nonhuman primate; pathogen; rRNA Genes; routine Bacterial stain

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Members of the Moraxella genus are gram-negative, aerobic bacteria that have been isolated from a variety of mammalian hosts. During a survey of bacteria that colonize the nasopharynx of rhesus macaques, isolates of the Moraxella genus, identified as M. catarrhalis, were observed in the majority of animals with demonstrated epistaxis, or "bloody nose syndrome." Several isolates from this survey were cryopreserved at the Tulane National Primate Research Center. One isolate was also obtained from an epistatic cynomolgus macaque at the U.S. Army Medical Research Institute of Infectious Diseases. Based on differences between the colony and gram stain morphology of rhesus isolates compared to that of human M. catarrhalis isolates, we hypothesized that the nonhuman primate Moraxella might be unique. These isolates were reassessed by multiple parameters. Despite differences in cell and colony morphology, the rhesus isolates, by each biochemical test, were identical to M. catarrhalis and not other human Moraxella subspecies. Biochemical analysis of the cynomolgus isolate by Vitek 2 compact (St Louis, Mo) revealed that it belonged to a Moraxella group, but could not differentiate between species. However, sequencing of the 16S ribosomal RNA gene from four representative rhesus isolates and the cynomolgus isolate showed closest homology to Moraxella lincolnii, a human respiratory tract inhabitant, with 90.16% identity. In order to examine rhesus macaques as potential hosts for M. catarrhalis, a total of eight animals were inoculated with human isolates of this strain. Only one of the animals was colonized and showed disease, whereas four of four macaques became epistatic after inoculation with the rhesus Moraxella isolate. Thus, this nasopharyngeal Moraxella species is a previously uncharacterized, distinct pathogen of nonhuman primates. It is not M. catarrhalis.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 莫拉氏菌属的成员是革兰氏阴性需氧细菌，已从多种哺乳动物宿主中分离出来。在对恒河猴鼻咽部定殖的细菌进行调查时，在大多数表现出鼻衄或“血鼻综合症”的动物中观察到莫拉氏菌属的分离株，被鉴定为粘膜炎莫拉氏菌。这次调查中的几个分离株被冷冻保存在杜兰国家灵长类动物研究中心。从美国陆军传染病医学研究所的一只上位食蟹猴中也获得了一种分离株。 根据恒河猴分离株与人类卡他莫拉菌分离株的菌落和革兰氏染色形态之间的差异，我们假设非人类灵长类莫拉氏菌可能是独特的。通过多个参数重新评估这些分离株。尽管细胞和菌落形态存在差异，但通过每次生化测试，恒河猴分离株与卡他莫拉菌相同，而与其他人类莫拉氏菌亚种不同。 Vitek 2 Compact（密苏里州圣路易斯）对食蟹猴分离物的生化分析显示，它属于莫拉氏菌属，但无法区分物种。 然而，对四种代表性恒河猴分离株和食蟹猴分离株的 16S 核糖体 RNA 基因进行测序，结果显示与人类呼吸道居民莫拉氏菌 (Moraxella lincolnii) 的同源性最接近，同一性为 90.16%。 为了检查恒河猴作为粘膜炎莫拉氏菌的潜在宿主，总共八只动物接种了该菌株的人类分离株。 只有一只动物被定植并表现出疾病，而四只猕猴中有四只在接种恒河猴莫拉氏菌分离株后变得上位。因此，这种鼻咽莫拉氏菌是一种以前未知的、独特的非人灵长类病原体。它不是卡他莫拉菌。