Tomographic X-Ray Microscope System

X射线断层显微镜系统

• 批准号: 8246978
• 负责人: Simon R Cherry
• 金额: $ 59.37万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-15 至 2013-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246978
• 关键词: 3-Dimensional; Animal Disease Models; Animal Model; Area; Arterial Fatty Streak; Atherosclerosis; Biocompatible Materials; Biological; Biopsy Specimen; Cell Culture Techniques; Cellularity; Complex; Core Facility; Data Set; Disease; Engineering; Funding; Genomics; Goals; Gold; Image; Imaging Techniques; Implant; Joints; Malignant Neoplasms; Mechanics; Methods; Microscope; Molecular; Morphology; Musculoskeletal Diseases; Names; Operative Surgical Procedures; Optics; Organ; Pathology; Patients; Phase; Property; Public Health; Regenerative Medicine; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Roentgen Rays; Secure; Source; Specimen; Staining method; Stains; Structure; System; Techniques; Technology; Therapeutic; Therapy Evaluation; Tissues; United States National Institutes of Health; X-Ray Tomography; bone imaging; calcification; density; detector; improved; in vivo; light scattering; nanodiagnostic; nanoparticle; nanorod; nanotherapeutic; reconstruction; regenerative therapy; scaffold; sensor; tumor

DESCRIPTION (provided by applicant): The goal of this proposal is to secure funding to add an X-ray "microscope" to the Center for Molecular and Genomic Imaging, a core facility at UC Davis that provides centralized infrastructure and expertise to conduct imaging studies in animal models and biospecimens. We propose to purchase an Xradia microXCT-200 high- resolution X-ray tomography microscope system that includes a 90kV microfocus X-ray source, a 4 M pixel CCD sensor, selectable detector optics with magnifications ranging from 4X to 40X, and a workstation for reconstruction and display of the large CT datasets. The system will be used for high resolution imaging of a range of biospecimens from 3-D cell culture, animal model tissues and patient biopsy samples in applications that span imaging of calcifications in tumors and atherosclerotic plaques, imaging of bone and joint pathologies and regenerative therapies, evaluation of the 3-D structure and mechanical properties of biomaterials and scaffolds, and imaging of the distribution of high-density (e.g. gold) nanoparticles and nanorods, to name just a few. The proposed system can achieve a spatial resolution (10% MTF) of < 1 ?m using the 40X optics and can image specimens up to 50 mm in size (20 mm with 40X detector). The system will be placed in our core facility and used to support and aid NIH-funded research in diverse areas such as musculoskeletal disease, atherosclerosis, cancer, regenerative medicine, biomaterials, nanodiagnostics and nanotherapeutics. The system will directly support improved understanding of disease through high-resolution, high contrast imaging of surgical or biopsy specimens from animal models of patients, the characterization of engineered implants, constructs and tissues, and the effects of a range of therapeutic strategies. PUBLIC HEALTH RELEVANECE: Imaging is a powerful technology for visualizing complex 3-D biological tissues and changes in these tissues that occur through disease. The proposed X-ray tomography microscope system utilizes advanced optics and phase contrast methods to achieve unprecedented resolution (~ 1 ¿m) and high contrast images across large volumes of biological tissue without staining or sectioning, providing undistorted and highly quantitative images of tissue morphology. This allows entire biopsy specimens, or organs/tissues from animal models to be imaged, and a wide range of morphological parameters (e.g. cellularity, density, vascularity, microarchitecture) to be assessed across the entire specimen, something that cannot be done with optical techniques due to the high degree of light scatter within biological tissues. The proposed X-ray microscope will be integrated in our imaging center with existing 2D and 3D in vivo imaging systems to provide a comprehensive resource for NIH funded researchers to study animal models of disease using imaging techniques.

描述（由应用程序提供）：该提案的目的是确保资金在分子和基因组成像中心添加X射线“显微镜”，UC Davis的核心设施提供了集中的基础架构和专业知识，以在动物模型和生物群中进行成像研究。我们建议购买Xradia microxct-20000高分辨率X射线断层扫描显微镜系统，其中包括90kV微型焦点X射线源，一个4 M Pixel CCD传感器，可选的探测器光学，具有4倍至40x的口量，以及用于重建和显示大型CT数据集的工作组。该系统将用于高分辨率成像，从3-D细胞培养，动物模型组织和患者活检样品中进行一系列生物测量，这些应用在涵盖肿瘤和动脉粥样硬化斑块中计算的成像，骨骼和关节病理学的成像以及再生疗法的成像，评估3-D结构和机械性能（评估生物形成的较高性和图像）以及对生物形成的分布以及图像的分布以及图像。黄金）纳米颗粒和纳米棒，仅举几例。所提出的系统可以使用40倍光学元件实现<1？m的空间分辨率（10％MTF），并且图像标本的大小高达50毫米（20毫米，带有40倍检测器）。该系统将放置在我们的核心设施中，并用于支持和协助NIH资助的研究，例如肌肉骨骼疾病，动脉粥样硬化，癌症，再生医学，生物材料，纳米诊断和纳米治疗学。该系统将通过高分辨率，对患者动物模型的外科手术或活检标本的高分辨率成像，工程不强制性，构造和组织的表征以及一系列治疗策略的影响来直接支持对疾病的了解。 公共卫生相关：成像是一种可视化复杂的3-D生物组织以及通过疾病发生的组织变化的强大技术。所提出的X射线断层扫描显微镜系统利用先进的光学和相对比方法来实现前所未有的分辨率（〜1�M）和大量生物组织的高对比度图像，而无需染色或切片，提供了组织形态学的未分配和高度定量的图像。这允许对动物模型的整个活检标本，或者来自动物模型的器官/组织进行成像，并且可以评估整个标本的广泛的形态参数（例如，细胞，密度，密度，血管，血管性，微体系结构），这些标本无法通过光散射在生物学组织内部的光学散射而无法完成。所提出的X射线显微镜将与现有的2D和3D体内成像系统中的成像中心集成在一起，为NIH资助的研究人员提供了使用成像技术研究疾病动物模型的全面资源。