Orbitrap Mass Spectrometer

Orbitrap 质谱仪

基本信息

批准号：
8247428
负责人：
Lauren Elizabeth Ball
金额：
$ 60万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-01 至 2013-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This proposal is for the addition of a Thermo Scientific LTQ Orbitrap Velos ETD hybrid FT mass spectrometer to the Mass Spectrometry Facility at the Medical University of South Carolina. The facility has a 39 year history of offerin state of the art mass spectrometry capability advancing a wide range of biomedical research interests in the local research community. Over the past three years, the facility has provided mass spectrometric analysis and assistance to 77 different faculty investigators within the university and multiple NIH-funded investigators at other institutions. The most highly requested services offered by the facility are protein identification and the characterization of posttranslational modifications. For these purposes, the most heavily used instruments in the facility are the low resolution linear ion trap mass spectrometers. The facility lacks an instrumen with the combined sensitivity, resolution, and multiple modes of peptide fragmentation that are achievable with the LTQ Orbitrap Velos ETD mass spectrometer. In fact, there is not an Orbitrap mass analyzer in South Carolina. The improved mass accuracy of this instrument will provide a higher level of confidence in peptide and protein identifications and in the site-assignment of posttranslational modifications (PTMs). Combining multiple fragmentation options (CID, HCD, ETD) with high mass accuracy measurements will enable quantitative approaches aimed at simultaneous identification and quantitation of protein modifications, a capability which is not currently available to facility users. The ETD ionization source accessory will 1) aid in projects requiring the characterization of PTMs and de novo sequencing and 2) provide new capabilities enabling top to middle-down protein analysis and quantitation of modifications that are destroyed during CID. The requested instrument will serve a representative group of Major Users including eleven NIH-funded investigators conducting research in the areas of cancer, cardiovascular disease, diabetes, vision, osteoporosis, and kidney disease. The Orbitrap Velos ETD instrument incorporates two mass analyzers and multiple fragmentation strategies yielding a powerful and very versatile instrument that will be well suited to address the broad range of protein analyses and characterization requested by investigators at MUSC and the neighboring universities and research centers in South Carolina. PUBLIC HEALTH RELEVANCE: The analysis of proteins by mass spectrometry is a fundamental analytical method utilized in biomedical research aimed at understanding processes underlying the onset and progression of disease and developing new therapeutics and diagnostics. The requested instrumentation will enhance our ability to identify and monitor changes in protein expression and regulatory post-translational modifications thereby advancing research in multiple areas including cancer, cardiovascular disease, diabetes, blindness, neurological disease, and osteoporosis.

描述（由申请人提供）：该提案是为了添加Thermo Scientific LTQ Orbitrap ETD Hybrid FT质谱仪到南卡罗来纳州医科大学的质谱设施。该设施具有39年的供应状态质谱能力的历史，这些能力推动了当地研究界的广泛生物医学研究兴趣。在过去的三年中，该设施为大学内的77名不同教职员工调查员提供了质谱分析和援助，以及其他机构的多个资助的研究人员。该设施提供的最高度要求的服务是蛋白质识别和翻译后修饰的表征。为了这些目的，设施中最常用的仪器是低分辨率线性离子陷阱质谱仪。该设施缺乏具有组合敏感性，分辨率和多种模式的肽片段化的仪器，可通过LTQ Orbitrap Velos ETD质谱仪来实现。实际上，南卡罗来纳州没有轨道质量分析仪。该仪器的质量精度提高将对肽和蛋白质鉴定以及翻译后修饰（PTMS）的位置分配提供更高的信心。将多个碎片选项（CID，HCD，ETD）与高质量精度测量相结合，将实现旨在同时识别和定量蛋白质修饰的定量方法，该方法目前尚不可用于设施使用者。 ETD电离源附件将1）帮助需要进行PTM和从头测序表征的项目以及2）提供新的功能，从而可以使中上下蛋白质分析以及对CID期间破坏的修饰进行定量。该请求的仪器将为一组代表性的主要用户提供服务，包括11名NIH资助的研究人员，他们在癌症，心血管疾病，糖尿病，视力，骨质疏松症和肾脏疾病方面进行研究。 Orbitrap Velos ETD仪器结合了两种质量分析仪和多种碎片化策略，产生了一种功能强大且非常多功能的仪器，该仪器将非常适合解决广泛的蛋白质分析和特征，并由MUSC的研究人员在南卡罗来纳州南卡罗来纳州的邻近大学和研究中心要求。公共卫生相关性：通过质谱分析蛋白质是一种基本的分析方法，旨在理解疾病发作和进展以及开发新的治疗疗法和诊断的过程。要求的仪器将增强我们识别和监测蛋白质表达和调节后翻译后修饰的变化的能力，从而在包括癌症，心血管疾病，糖尿病，失明，神经疾病和骨质疏松症在内的多个地区进行研究。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

S-Glutathionylated Serine Proteinase Inhibitors as Biomarkers for Radiation Exposure in Prostate Cancer Patients.

S-谷胱甘肽化丝氨酸蛋白酶抑制剂作为前列腺癌患者辐射暴露的生物标志物。

DOI：
10.1038/s41598-019-50288-9
发表时间：
2019
期刊：
Scientific reports
影响因子：
4.6
作者：
Zhang,Leilei;Zhang,Jie;Ye,Zhiwei;Manevich,Yefim;Townsend,DanyelleM;Marshall,DavidT;Tew,KennethD
通讯作者：
Tew,KennethD

In Situ Imaging of Tryptic Peptides by MALDI Imaging Mass Spectrometry Using Fresh-Frozen or Formalin-Fixed, Paraffin-Embedded Tissue.