Proteomics Core

蛋白质组学核心

基本信息

批准号：
10460362
负责人：
Lauren Elizabeth Ball
金额：
$ 27.18万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-01 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10460362
关键词：
Acetylation Address Advanced Development Applications Grants Arginine Award Bioinformatics Biological Markers Biology Cancer Center Support Grant Cells Centers of Research Excellence Complement Computer software Consult Consultations Custom Cysteine Data Analyses Detection Development Disease Disease Progression Dissociation Drug resistance Education Electron Transport Ensure Enzymes Equilibrium Evaluation Experimental Designs Faculty Freezing Goals Grant Human Resources Image Infrastructure Investments Lysine Mass Spectrum Analysis Methodology Modification National Institute of General Medical Sciences Oxidants Oxidation-Reduction Oxides Peptides Phase Positioning Attribute Post-Translational Modification Site Post-Translational Protein Processing Preparation Proline Proteins Proteomics Pyruvaldehyde Reproducibility Research Research Personnel Resolution Resources Sampling Serine/Threonine Phosphorylation Services Signal Transduction Site Slide South Carolina Spatial Distribution Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Stress Sulfonic Acids System Technology Tissue imaging Tissues Training Treatment Efficacy Tyrosine Ubiquitin United States National Institutes of Health Update base computerized data processing cost crosslink data acquisition disulfide bond experience experimental study glycation glycosylation imaging capabilities improved in vivo instrument instrumentation mass spectrometric imaging nano new technology novel oxidation programs protein expression response success therapy resistant translational study

项目摘要

Proteomics Core – Project Summary The broad objective of the Proteomics Core in the SC COBRE in Oxidants, Redox Balance, and Stress Signaling (Redox COBRE) is to provide state-of-the-art, mass spectrometry (MS)-based proteomic capabilities to enable elucidation of redox signaling mechanisms underlying disease, therapeutic efficacy of cells and drugs, and therapeutic resistance. The Core provides expertise, technical assistance, instrumentation, and interpretation of analytical results for quantitative proteomic experiments and the characterization of redox-sensitive post- translational modifications. During Phase I and II, acquisition of the Orbitrap Elite ETD MS and Orbitrap Fusion Lumos ETD/UVPD MS nano-LC-MS/MS systems (NIH S10 OD010731 and S10 OD025126, PI: Lauren Ball) permitted detection of challenging modifications using complementary peptide fragmentation approaches (ETD and EThcD) and the development of advanced, quantitative proteomic workflows as needed by COBRE investigators. During Phase III, the Core will further develop and update these capabilities to reflect rapid advances in instrumentation, software, and methodologies relevant to redox proteomics. Additionally, in response to the needs of COBRE-affiliated investigators, proteomic tissue imaging capabilities using MALDI- Imaging MS (IMS) will be provided to discern the spatial distribution of enzymatically-digested peptides within tissue biospecimens on slides for the discovery of putative biomarkers and mechanisms underlying disease progression. Detection of disease-specific proteins and redox-sensitive post-translational modifications by LC- MS/MS and IMS has raised the need for targeted proteomic capabilities to verify these findings and further advance translational studies. Providing this complement of proteomic technologies will build on the successes of the Core and position the Core to establish a comprehensive, independent resource. The Core has leveraged previous investments made by the NIGMS during Phase I and II of the COBRE program, the OD and NIGMS for Shared Instrumentation Grants, the NCI Cancer Center Support Grant, and long-standing, strong institutional commitment to develop into a successful institutional resource critical to the needs of Redox COBRE investigators. At present there are 19 COBRE-affiliated users of the Proteomics Core that are PIs of 15 active NIH awards that rely on the LC-MS/MS capabilities, five of these awards also depend on IMS capabilities.

蛋白质组学核心 - 项目摘要蛋白质组学核心在氧化剂，氧化还原平衡和应力信号中的蛋白质组学核心的广泛目标（氧化还原胶）将提供最先进的质谱法（MS）的蛋白质组学能力以启用阐明氧化还原信号传导机制，细胞和药物的治疗疗效以及药物和药物。治疗性抗性。定量蛋白质组学实验的分析结果以及对氧化还原敏感后的表征翻译的修改。 Lumos ETD/UVPD MS Nano-LC-MS/MS系统（NIH S10 OD010731和S10 OD25126，PI：LAUREN BALL）允许使用竞争片段碎片片段化方法（ETD）检测具有挑战性的修改（ETD）和ETHCD）以及根据需要的高级定量蛋白质组学工作流的开发研究人员。与氧化还原蛋白质组学相关的仪器，软件和方法的进步响应与毛核相关研究者的需求，蛋白质组织成像能力使用MALDI- 成像MS（IMS）将被证明辨别酶消化肽的空间分布幻灯片上的组织生物测量，以发现假定的生物标志物和基础疾病的机制疾病特异性蛋白和对氧化还原敏感的翻译后修饰的进展 MS/MS和IMS IMS提出了忘记的蛋白质组学cability的需求，以验证发现和进一步提前的翻译研究。核心和核心的定位，以建立全面的独立资源。在山毛计划的第一阶段和第二阶段，奈克的先前投资，OD和Nigms的先前投资共享的仪器赠款，NCI癌症中心支持补助金以及长期存在的强大研究所致力于发展成为氧化还原需求至关重要的成功研究所的资源目前。 NIH依靠LC-MS/MS功能的奖项，其中五个奖项也取决于IMS能力。